Targeted Metabolomics

Targeted Metabolomics — Absolute Quantification Panels

Absolute quantification against a defined metabolite list: isotope-internal-standard calibration with standard-curve quantification, delivered as absolute concentrations (e.g., ng/mL or ng/g). The service spans 30+ mature panels—amino acids, bile acids, fatty acids, glucose metabolism, neurotransmitters, vitamins, hormones and more—with on-demand customization, making it the standard solution for mechanism research, biomarker validation and large-cohort quantification.

30+
mature targeted panels
READY-MADE PANELS
MRM
Isotope-IS Absolute Quantification
ABSOLUTE QUANTIFICATION
1–3 weeks
standard turnaround for mature panels
STANDARD TURNAROUND

Technical Specifications

TECHNICAL SPECIFICATIONS
PlatformUltra-high-performance liquid chromatography–triple-quadrupole mass spectrometry (UHPLC-MS/MS) and GC-MS
QuantificationIsotope-internal-standard calibration + standard-curve absolute quantification
Quantification UnitAbsolute concentration units such as ng/mL and ng/g
QCPooled QC samples inserted across the batch to monitor signal drift and stability

Sample Requirements

SAMPLE REQUIREMENTS
Serum / Plasma
50 μL
Tissue
30 mg
Cells
1–5 × 10⁶ cells
Feces / Gut Content
100 mg fresh / 20 mg lyophilized
Urine
50 μL
Exosomes
50 μL
Replicates: ≥6 per group for cell / microbial samples; ≥10 per group for animal samples; ≥50 per group for clinical samples with strictly controlled inclusion criteria.
Submission notes: recommended amounts are per single assay—please submit 2–3× the recommended amount; ship on dry ice or cold chain; sample requirements vary slightly by panel—follow the project plan.

Workflow

WORKFLOW
1
Consultation
Research objective / panel selection
2
Proposal & Quote
Targets / timeline / agreement
3
Sample Intake
Cold chain / pre-check / labeling
4
Experiment
Acquisition / QC
5
Delivery
Quantification + interpretation + raw data
Deliverables: absolute-quantification table for each metabolite · group-difference analysis · PCA / clustering · KEGG pathway enrichment · full analysis report and raw data.

FAQ

FAQ
How long does a Targeted Metabolomics project take?
Mature targeted panels typically take 1-3 weeks; untargeted projects take 2-4 weeks (counted from sample receipt and QC approval). The exact timeline is confirmed in writing before project start, and progress is trackable on the OmicsHub cloud platform.
What if my sample amount is below the recommendation?
Please do not dilute your samples. Low-input samples can often be accommodated by adjusting the preparation workflow — tell us the actual amount and our technical team will assess feasibility and note adjustments in the study plan.
Can I add targets to an existing panel?
Yes. Mature panels support on-demand additions; novel analytes outside the list can be implemented through custom method development (requires standards and extra validation time) — see our Custom Targeted Metabolomics service.
What samples do you need for Targeted Metabolomics?
Serum/plasma ≥100 μL (fasting, no haemolysis), tissue ≥50 mg (snap-frozen), cells ≥1×10⁶ (medium washed off), urine ≥200 μL. Keep fasting time consistent, aliquot into single-use volumes and avoid freeze–thaw. Full guidance: Technical Resources → Sample Guide.

Delivery timing: sample shipping, customs declaration and similar steps may extend turnaround; actual project timelines prevail.

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