Alkaloids Panel

Targeted List & Analysis Examples

Targeted List (partial)

NO.	Chinese Name	English Name	CAS No.
1	乌头原碱	Aconine	509-20-6
2	乌头碱	Aconitine	302-27-2
3	阿托品	Atropine	51-55-8
4	小檗碱	Berberine	2086-83-1
5	喜树碱	Camptothecin	2114454
6	长春质碱	Catharanthine	2468-21-5
7	辛可宁	Cinchonine	118-10-5
8	秋水仙碱	Colchicine	64-86-8

Full list available on request — contact our team.

Technical Specifications

TECHNICAL SPECIFICATIONS

Platform	UHPLC coupled with triple-quadrupole MS (UHPLC-MS/MS)
Ion Mode	Positive/negative ion switching acquisition
Quantification Unit	Standard-curve absolute quantification, ng/mL or ng/g
QC	Pooled QC samples inserted across the batch to monitor signal drift and stability

Sample Requirements

SAMPLE REQUIREMENTS

Leaf / Stem

100 mg

Flower / Pollen

50 mg

Root / Rhizome

100 mg

Seed / Fruit

200 mg

Callus / Mycelium

200 mg

Lyophilized Powder / Extract

100 mg dry weight

Replicates: cell / microbial samples ≥6 replicates per group; animal samples ≥10 per group; clinical samples ≥50 per group with strictly controlled inclusion criteria. Sampling site, timing and handling must be consistent between experimental and control groups.

Shipping notes: applicable to serum/plasma, various tissues, feces and gut content, urine, saliva, cells / cell culture medium and more. Recommended amounts are for a single experiment — please ship 2–3× the recommended amount; keep sampling sites consistent across groups for tissue samples; ship on dry ice or cold chain.

Workflow

WORKFLOW

Consultation

Study goals / panel selection

Proposal & Quote

Targets / timeline / agreement

Sample Intake

Cold chain / pre-check / labeling

Experiment

Acquisition / QC

Delivery

Quantification + interpretation + raw data

Analysis deliverables: alkaloid type composition, toxic-component levels and statistics · absolute quantification table for all metabolites (abundance + units) · intergroup T-test with fold change · PCA / PLS-DA modeling and hierarchical clustering · KEGG annotation and pathway enrichment · full analysis report and raw data.

Analysis Examples

ANALYSIS EXAMPLES

Example figures are generated from real project data; actual figures and analyses depend on the detection panel and study plan.

T-test Bar Chart

All metabolites sorted by ascending p-value; grouped bars show group means ± SD with p-values annotated beside the bars.

Hierarchical Clustering Heatmap

Z-score normalized abundance patterns of all metabolites; rows ordered by hierarchical clustering — red for high, blue for low abundance.

Disease Association Bar Chart

Known metabolite–disease associations mined from HMDB and other databases, ranked by the number of associated metabolites.

Metabolite Abundance Analysis

Faceted boxplots of significantly different metabolites between groups; intergroup comparison by Welch's t-test.

Bar Chart Analysis

Per-sample bar charts of differential metabolites, visualizing group differences and individual dispersion.

References

REFERENCES

[1] VANDERPLANCK M, GLAUSER G. Integration of non-targeted metabolomics and automated determination of elemental compositions for comprehensive alkaloid profiling in plants[J]. Phytochemistry, 2018, 154: 1-9.

[2] AHMAD L, FAN M, ZEMEDE J, et al. Recent Advances in Analytical Methods for Aconitine Alkaloid in Natural Products: A Systematic Review[J]. Phytochemical Analysis, 2025, 36(6): 1621-1648.

Applications

APPLICATIONS

Active Ingredients of Medicinal PlantsMaterial Basis of TCM EfficacyAlkaloid Toxicology EvaluationNatural-Product Drug DiscoveryPlant Defense Metabolism

FAQ

How long does a Alkaloids Panel project take?

Mature targeted panels typically take 1-3 weeks; untargeted projects take 2-4 weeks (counted from sample receipt and QC approval). The exact timeline is confirmed in writing before project start, and progress is trackable on the OmicsHub cloud platform.

What if my sample amount is below the recommendation?

Please do not dilute your samples. Low-input samples can often be accommodated by adjusting the preparation workflow — tell us the actual amount and our technical team will assess feasibility and note adjustments in the study plan.

Can I add targets to an existing panel?

Yes. Mature panels support on-demand additions; novel analytes outside the list can be implemented through custom method development (requires standards and extra validation time) — see our Custom Targeted Metabolomics service.

What samples do you need for Alkaloids Panel?

Serum/plasma ≥100 μL (fasting, no haemolysis), tissue ≥50 mg (snap-frozen), cells ≥1×10⁶ (medium washed off), urine ≥200 μL. Keep fasting time consistent, aliquot into single-use volumes and avoid freeze–thaw. Full guidance: Technical Resources → Sample Guide.