PRM TARGETED

PRM Proteomics

Parallel Reaction Monitoring — Targeted Protein Quantification

Orbitrap PRM (parallel reaction monitoring) targets proteotypic peptides of candidate proteins, with heavy-labeled peptides enabling relative/absolute quantification. High selectivity, high reproducibility and antibody-free — an ideal alternative to high-throughput Western blot for candidate biomarker validation, closing the "discovery → validation" loop with discovery proteomics.

Technical Specifications

TECHNICAL SPECIFICATIONS

Platform	Orbitrap high-resolution MS in PRM mode
Quantification	Heavy-peptide internal standards; relative / absolute quantification
Throughput	Dozens to hundreds of peptides monitored per injection
Integration	Directly accepts candidate lists from discovery proteomics or literature

Sample Requirements

SAMPLE REQUIREMENTS

Cells

≥ 1 × 10⁶ cells

Tissue

≥ 50 mg

Serum / Plasma

≥ 20 μL

Replicates: ≥3 biological replicates per group; larger sample sizes recommended for validation cohorts.

Shipping notes: store at -80°C and ship on dry ice; provide the candidate protein list (UniProt IDs) for peptide design.

Workflow

WORKFLOW

List Confirmation

Candidates / peptide design

Proposal & Quote

Peptide count / timeline / agreement

Sample Intake

Pre-check / digestion

PRM Acquisition

Targeted monitoring / QC

Delivery

Quantification + analysis report

FAQ

How long does a PRM Proteomics project take?

Standard proteomics projects take 3-5 weeks depending on sample number and analysis depth; key milestones (sample QC, LC-MS/MS completion) are proactively synced with you.

How should proteomics samples be stored and shipped?

Store at -80°C and ship on dry ice; avoid repeated freeze-thaw cycles. Protease inhibitors are recommended for protein samples. A tailored Sample Collection Guide is provided before shipping.

How can DIA proteomics results be validated?

We recommend PRM targeted proteomics for candidate validation (antibody-free, highly reproducible), or classic assays such as Western Blot and ELISA, forming a "discovery → validation" closed loop.

What samples do you need for PRM Proteomics?

Cells ≥1×10⁶, tissue ≥50 mg, serum/plasma ≥50 μL, FFPE ≥4 sections (5 μm). Protein samples tolerate freeze–thaw poorly — aliquot so each tube is thawed once; for PTM work include matched controls and avoid phosphate buffers. See Technical Resources → Sample Guide.