PTM Proteomics

Post-translational Modification Proteomics

For post-translational modifications — phosphorylation, lactylation, ubiquitination, acetylation, palmitoylation and more — modification-specific enrichment is followed by high-resolution MS acquisition, delivering both site identification and modification-level quantification. Site motif analysis and kinase–substrate inference are supported, making this a core approach for signaling, epigenetic regulation and drug-mechanism research.

Technical Specifications

TECHNICAL SPECIFICATIONS

Platform	Orbitrap Astral / timsTOF high-resolution MS
Enrichment	TiO₂ / IMAC / antibody-based enrichment (selected by PTM type)
Analysis Content	Site identification, site quantification, motif analysis, kinase–substrate association
Deliverables	Raw data + standard analysis report + OmicsHub cloud archiving

Sample Requirements

SAMPLE REQUIREMENTS

Cells

≥ 1 × 10⁷ cells

Tissue

≥ 100 mg

Protein Lysate

≥ 2 mg

Replicates: ≥3–4 biological replicates per group; 4 or more recommended for robust statistics.

Shipping notes: store at -80°C and ship on dry ice; avoid freeze-thaw cycles. Modified proteins degrade easily — minimize processing time and add appropriate enzyme inhibitors.

Workflow

WORKFLOW

Consultation

PTM type / study goals

Proposal & Quote

Enrichment strategy / timeline / agreement

Sample Intake

Pre-check / protein assay

Enrichment & Acquisition

PTM enrichment / high-res acquisition

Delivery

Site table + analysis report

FAQ

How long does a PTM Proteomics project take?

Standard proteomics projects take 3-5 weeks depending on sample number and analysis depth; key milestones (sample QC, LC-MS/MS completion) are proactively synced with you.

How should proteomics samples be stored and shipped?

Store at -80°C and ship on dry ice; avoid repeated freeze-thaw cycles. Protease inhibitors are recommended for protein samples. A tailored Sample Collection Guide is provided before shipping.

How can DIA proteomics results be validated?

We recommend PRM targeted proteomics for candidate validation (antibody-free, highly reproducible), or classic assays such as Western Blot and ELISA, forming a "discovery → validation" closed loop.

What samples do you need for PTM Proteomics?

Cells ≥1×10⁶, tissue ≥50 mg, serum/plasma ≥50 μL, FFPE ≥4 sections (5 μm). Protein samples tolerate freeze–thaw poorly — aliquot so each tube is thawed once; for PTM work include matched controls and avoid phosphate buffers. See Technical Resources → Sample Guide.