Flow Cytometry

Flow Cytometry

Flow Cytometry Analysis Services

Rapid, quantitative single-cell analysis of cell populations by multicolor flow cytometry, covering apoptosis, cell cycle, immunophenotyping, and surface and intracellular marker detection—delivering reliable cell-phenotype data for immunology, oncology and cell-biology research.

Assay Menu

ASSAY MENU

Item	Notes
Apoptosis	Annexin V / PI double staining to distinguish early- and late-stage apoptosis
Cell cycle	PI staining to analyze G0/G1, S and G2/M phase distribution
Immunophenotyping	Surface-marker analysis of T / B / NK and other immune-cell subsets
Intracellular factors	Detection of intracellular cytokines and phosphorylated proteins

Sample Requirements

SAMPLE REQUIREMENTS

Cell suspension

≥ 1 × 10⁶ cells/mL

Peripheral blood

≥ 1 mL (anticoagulated)

Tissue

Process fresh tissue promptly

Important: flow cytometry depends on live-cell condition—samples must be freshly prepared and kept cold throughout; please confirm the time window with our lab before shipping.

Submission notes: ship cell samples with culture medium on ice packs; ship anticoagulated blood at room temperature for delivery within 24 hours.

Workflow

WORKFLOW

Consultation

Assay items / antibody panel

Proposal & Quote

Targets / timeline / agreement

Sample Intake

Viability assessment / counting

Staining & acquisition

antibody staining / data acquisition

Delivery

gating analysis + statistical charts

FAQ

How long does a Flow Cytometry project take?

Standard projects take 1-3 weeks depending on complexity and sample size; projects involving cell modeling or multi-batch processing follow the confirmed study plan.

Can we provide our own reagents or antibodies?

Yes. You may supply key reagents/antibodies, or we can purchase them per the study plan. Please include datasheets and storage conditions for supplied materials.

Does the report include raw data?

Yes. Deliverables include raw records (membrane images, raw values, image files) together with processed statistical results — fully traceable.

What samples do you need for Flow Cytometry?

ELISA serum/plasma ≥50 μL, WB lysate ≥20 μg protein, flow samples ≥100 μL (within 24 h), tissue ≥0.5 cm³ (4% PFA 24–48 h; OCT-embedded, dry ice). See Technical Resources → Sample Guide.