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DIA Proteomics

DIA Proteomics — Discovery to Targeted Validation

Centered on high-speed Orbitrap Astral acquisition, DIA discovery quantification connects seamlessly with PRM targeted validation — closing the "discovery → validation" loop at the protein level. Deliverables follow the same standard as metabolomics: raw data + standard analysis report + OmicsHub cloud archiving, with shared sample systems and shipping guidelines.

Orbitrap Astral
High-Speed Acquisition
HIGH-SPEED ACQUISITION
6
Proteomics Services
PROTEOMICS SERVICES
DIA + PRM
Discovery-to-Validation Loop
DISCOVERY TO VALIDATION

Service Items

PROTEOMICS SERVICES
Deliverables：raw data + standard analysis report + OmicsHub cloud archiving; sample systems and shipping guidelines are shared with metabolomics; differential protein lists from DIA proteomics feed directly into PRM validation.

Analysis Examples

SELECTED FIGURES FROM ACTUAL REPORTS
Sample Intensity Density Plot
Sample Intensity Density Plot
log10 density curves of protein quantification intensities per sample, examining dynamic range and overall inter-sample consistency.
Sample Correlation Heatmap (PCC)
Sample Correlation Heatmap (PCC)
Pairwise Pearson correlation heatmap of all samples — higher within-group correlation and clearer intergroup separation indicate more reliable data.
Sample Intensity Violin Plot
Sample Intensity Violin Plot
Per-sample protein intensity distributions with box overlays, for detecting outliers and checking normalization.
Protein Abundance Plot
Protein Abundance Plot
Relative abundance comparison of target proteins across groups, with error bars and test p-values.
Differential Protein Radar Chart
Differential Protein Radar Chart
Radar chart of fold changes for differential proteins across comparisons, visually comparing magnitude and pattern.
Differential Protein Correlation
Differential Protein Correlation
Pairwise correlation matrix of differential proteins, identifying co-regulated protein modules.
Volcano Plot
Volcano Plot
log2 FC on the x-axis and −log10(p) on the y-axis, presenting fold change and significance together, with up/down-regulated protein counts labeled.
Multi-Group KEGG Enrichment Heatmap
Multi-Group KEGG Enrichment Heatmap
Clustered heatmap of KEGG pathway −log10(p) across comparisons, comparing pathway responses between groups.
PPI Network
PPI Network
Interaction network of differential proteins; node size encodes connectivity, edges distinguish interaction strength and direction.
KEGG Enrichment Pathway Map
KEGG Enrichment Pathway Map
Differential proteins marked on KEGG pathway maps, visualizing pathway-level changes.
GO Enrichment Bar Chart
GO Enrichment Bar Chart
Proportions of enriched proteins across the three GO domains (biological process / cellular component / molecular function).
GO Enrichment Bubble Plot
GO Enrichment Bubble Plot
Bubble plot of GO-term enrichment; bubble size encodes protein counts, color intensity encodes p-values.

Applications

APPLICATIONS
Differential Proteins & BiomarkersLarge-Cohort Proteome AtlasesDisease Subtyping & PrognosisDrug Target DiscoveryBlood Protein Biomarkers

FAQ

FAQ
How long does a DIA Proteomics project take?
Standard proteomics projects take 3-5 weeks depending on sample number and analysis depth; key milestones (sample QC, LC-MS/MS completion) are proactively synced with you.
How should proteomics samples be stored and shipped?
Store at -80°C and ship on dry ice; avoid repeated freeze-thaw cycles. Protease inhibitors are recommended for protein samples. A tailored Sample Collection Guide is provided before shipping.
How can DIA proteomics results be validated?
We recommend PRM targeted proteomics for candidate validation (antibody-free, highly reproducible), or classic assays such as Western Blot and ELISA, forming a "discovery → validation" closed loop.
What samples do you need for DIA Proteomics?
Cells ≥1×10⁶, tissue ≥50 mg, serum/plasma ≥50 μL, FFPE ≥4 sections (5 μm). Protein samples tolerate freeze–thaw poorly — aliquot so each tube is thawed once; for PTM work include matched controls and avoid phosphate buffers. See Technical Resources → Sample Guide.

Related Services

RELATED SERVICES

PTM Proteomics

PTM Proteomics

4D Proteomics

4D Proteomics

PRM Proteomics

PRM Proteomics

Delivery timing: sample shipping, customs declaration and similar steps may extend turnaround; actual project timelines prevail.

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