SINGLE-CELL

Single-cell RNA-seq

Single-cell RNA Sequencing (scRNA-seq)

Built on dual platforms—10x Genomics Chromium X and MGI DNBelab—using microfluidic droplet technology for high-throughput single-cell capture and transcriptome library preparation, resolving cellular heterogeneity at single-cell resolution: cell clustering and annotation, differential expression, pseudotime and cell–cell interaction analysis. Suited to tumor-microenvironment, immunology, developmental and neuroscience research.

Technical Specifications

TECHNICAL SPECIFICATIONS

Capture platform	10x Genomics Chromium X / MGI DNBelab C-TaiM 4
Sequencing Platform	Illumina high-throughput sequencing (PE150)
Analysis Content	Cell clustering and annotation, differential expression, pseudotime, cell–cell communication
Key QCs	Suspension viability, cell concentration and capture counts assessed throughout

Sample Requirements

SAMPLE REQUIREMENTS

Single-cell suspension

viability ≥ 80%

Cell concentration

700–1200 cells/μL

Fresh tissue

deliver ASAP on cold chain

Key note: single-cell experiments are highly sensitive to sample freshness—book instrument time with our lab in advance and complete capture as soon as the suspension is prepared.

Submission notes: ship fresh tissue on ice packs for delivery within 4 hours; keep cell suspensions ice-cold throughout; we recommend discussing the dissociation plan in advance.

Workflow

WORKFLOW

Consultation

tissue type / research objective

Proposal & Quote

cell count / timeline / agreement

Suspension prep

dissociation / viability QC

Capture & sequencing

library prep / sequencing / QC

Analysis delivery

clustering & annotation + analysis report

FAQ

How long does a Single-cell RNA-seq project take?

From sample QC approval, standard timelines are 3-6 weeks (library prep, sequencing and bioinformatics included); large cohorts or deep custom analyses follow the confirmed study plan.

What if my samples fail QC?

Failed QC is reported immediately with reasons, and we discuss resampling or plan adjustments with you — sequencing never proceeds without qualification.

What bioinformatics is included? Can I add custom analyses?

Standard delivery covers QC, mapping/assembly, quantification, differential and functional enrichment analyses. Custom deep-dive and multi-omics integrative analyses are supported on request.

What samples do you need for Single-cell RNA-seq?

Total RNA ≥1 μg with RIN ≥7 (snap-frozen, dry ice); microbial and faecal samples ≥200 mg (sterile, −80 °C); whole blood for WGS ≥500 μL (EDTA only, never heparin). RNA integrity matters more than concentration. See Technical Resources → Sample Guide.