ELISA ASSAY SERVICE

ELISA

Enzyme-Linked Immunosorbent Assay — 1,003 Unique Targets

Enzyme-linked immunosorbent assay (ELISA) is based on specific antigen–antibody binding: target proteins, hormones or small molecules are captured on a solid phase, developed with an enzyme-labeled secondary antibody, and absolutely quantified by colorimetry. Sandwich ELISA suits large protein analytes, while competitive-inhibition ELISA suits small molecules and hormones — two complementary systems. Kits come in 96T / 48T formats, and multiple analytes can be assayed in parallel on the same sample batch.

1,003
Detectable Analytes
UNIQUE TARGETS
16
Analyte Categories
ASSAY CATEGORIES
96T / 48T
Two Kit Formats
KIT FORMATS

Specifications & Samples

TECHNICAL SPECIFICATIONS
ItemDetails
MethodologySandwich ELISA (large proteins) / competitive-inhibition ELISA (small molecules and hormones) — two complementary systems
Kit Formats96T (~80 samples) / 48T (~40 samples); orders can be combined by analyte count and sample size
Applicable SamplesRoutine samples including serum, plasma, tissue homogenate, cell culture supernatant, urine and feces; sampling kits and dry-ice shipping guidance provided
DeliverablesConcentrations calculated from standard curves, delivered with raw OD values

Species Coverage

SPECIES COVERAGE
Human · HUMAN
740+ kits covering 710+ analytes
Mouse · MOUSE
520+ kits covering 510+ analytes
Rat · RAT
440+ kits covering 430+ analytes

Workflow

SERVICE WORKFLOW
1
Consultation
Study goals / design
2
Proposal & Quote
Targets / timeline / agreement
3
Sample Intake
Cold chain / pre-check / labeling
4
Experiment
Acquisition / QC
5
Delivery
Data + report + interpretation

Analyte Categories & Representative Markers

ASSAY MENU
CategoryItemsRepresentative Analytes
Cytokines & Chemokines247Interleukin family (IL-1β, IL-6, IL-10, IL-17A, etc.), TNF-α/β/γ, CCL and CXCL chemokines and their receptors
Immunoglobulins · Complement147Immunoglobulins G / M / A (41 IgG items, 16 IgM items), complement C3 / C4, antiphospholipid antibodies, anti-β2 glycoprotein 1 antibodies
Surface Markers & Adhesion Molecules103CD molecules (CD3 / CD4 / CD8 / CD14 / CD19 / CD25 / CD34 / CD44 / CD68, etc.) and adhesion molecules (ICAM / VCAM, etc.)
Endocrinology & Hormones9125-hydroxyvitamin D, triiodothyronine (T3), FSH, testosterone (T), estradiol (E2), progesterone, aldosterone
Coagulation & Fibrinolysis75Coagulation factors I–XIII (FⅧ / FⅨ / FⅩ / FXIII A, etc.), von Willebrand factor (vWF), t-PA, PAI-1
Growth Factors & Receptors63IGF-1 / IGF-2, VEGF-A~D, FGF, TGF-β1~β4
Vitamins & Minerals4825(OH)D2 / 25(OH)D3, vitamin B12, folate, ferritin, transferrin, Klotho protein
Pathogen Antigens & Antibodies43Hepatitis B surface / e / core antigens (HBsAg, HBeAg, HBcAg), HCV, HIV, cytomegalovirus, endotoxin
Signaling & Stress Proteins41Toll-like receptors TLR1–TLR9, heat-shock proteins HSP-27 / HSP-70 / HSP-90, AKT, WNT family
Cardiovascular & Endothelium33hs-cardiac troponin T / I, endothelin-1 (ET-1), Lp-PLA2, 6-keto-PGF1α, angiotensin
Glucose Metabolism & Insulin31Insulin (INS), C-peptide, proinsulin, anti-insulin and insulin-receptor antibodies, IGF-binding proteins
Neurotransmitters & Neural Injury Markers24Serotonin (5-HT), BDNF, S100B, Tau and total Tau, amyloid-β Aβ1-40 / Aβ1-42
Lipid Metabolism & Adipokines24Apolipoproteins A / B / E and ApoA5, leptin (LEP), adiponectin (ADPN), Lp-PLA2
Tumor Markers23CEA, CA19-9 / CA125 / CA15-3, AFP, PSA / fPSA
Bone Metabolism & ECM21Osteocalcin (BGP/OCN), β-CTx, C-terminal telopeptides of type I/II collagen, hyaluronic acid (HA)
Apoptosis13Caspase-1 / Caspase-3 / Caspase-8 / Caspase-9 and their precursor fragments

Applications

APPLICATIONS
Cytokine & Inflammation QuantificationHormone & Marker TestingDrug Concentration AssaysImmunology ValidationTargeted Validation of Omics Hits

FAQ

FAQ
How long does a ELISA project take?
Standard projects take 1-3 weeks depending on complexity and sample size; projects involving cell modeling or multi-batch processing follow the confirmed study plan.
Can we provide our own reagents or antibodies?
Yes. You may supply key reagents/antibodies, or we can purchase them per the study plan. Please include datasheets and storage conditions for supplied materials.
Does the report include raw data?
Yes. Deliverables include raw records (membrane images, raw values, image files) together with processed statistical results — fully traceable.
What samples do you need for ELISA?
ELISA serum/plasma ≥50 μL, WB lysate ≥20 μg protein, flow samples ≥100 μL (within 24 h), tissue ≥0.5 cm³ (4% PFA 24–48 h; OCT-embedded, dry ice). See Technical Resources → Sample Guide.

Delivery timing: sample shipping, customs declaration and similar steps may extend turnaround; actual project timelines prevail.

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