Western Blot

Western Blot — Protein Expression Validation

A full Western-blot workflow—from protein extraction and quantification to electrophoresis, transfer, antibody incubation and imaging analysis—providing qualitative / semi-quantitative verification of target-protein expression level and molecular weight; a classic approach for gene-function studies and proteomics-result validation.

Service Scope

SERVICE SCOPE

Step	Notes
Protein extraction & quantification	Total-protein extraction from tissue/cells with BCA quantification
Electrophoresis & transfer	SDS-PAGE separation with wet / semi-dry transfer
Antibody incubation	Primary (purchasable on request) and secondary antibody incubation with ECL development
Imaging & analysis	Chemiluminescence imaging with housekeeping-normalized densitometry

Sample Requirements

SAMPLE REQUIREMENTS

Cells

≥ 1 × 10⁶ cells

Tissue

≥ 50 mg

Total protein

≥ 100 μg

Replicates: ≥3 biological replicates per group to ensure valid semi-quantitative statistics.

Submission notes: store samples at -80°C and ship on dry ice; provide the target-protein name, molecular weight and species; primary antibodies may be supplied by you or purchased by us.

Workflow

WORKFLOW

Consultation

Target protein / antibody confirmation

Proposal & Quote

Targets / timeline / agreement

Sample Intake

Pre-check / protein assay

Experiment

electrophoresis / transfer / exposure

Delivery

blot images + densitometric statistics

FAQ

How long does a Western Blot project take?

Standard projects take 1-3 weeks depending on complexity and sample size; projects involving cell modeling or multi-batch processing follow the confirmed study plan.

Can we provide our own reagents or antibodies?

Yes. You may supply key reagents/antibodies, or we can purchase them per the study plan. Please include datasheets and storage conditions for supplied materials.

Does the report include raw data?

Yes. Deliverables include raw records (membrane images, raw values, image files) together with processed statistical results — fully traceable.

What samples do you need for Western Blot?

ELISA serum/plasma ≥50 μL, WB lysate ≥20 μg protein, flow samples ≥100 μL (within 24 h), tissue ≥0.5 cm³ (4% PFA 24–48 h; OCT-embedded, dry ice). See Technical Resources → Sample Guide.