Widely Targeted Metabolomics

Technical Specifications

TECHNICAL SPECIFICATIONS

Item	Details
Platform	UHPLC coupled with triple-quadrupole MS (UHPLC-MS/MS) · MRM mode with polarity switching
Quantification	Isotope internal standards + calibration-curve absolute quantification
Chromatography	20-minute gradient per injection
QC	QC samples inserted across the batch to monitor signal drift
Biological Replicates	Cell / microbial samples ≥6 replicates per group; animal samples ≥10 per group; clinical samples ≥50 per group with strictly controlled inclusion criteria. Sampling site, timing and handling must be consistent between experimental and control groups.

Sample Requirements

SAMPLE REQUIREMENTS

Serum / Plasma

50 μL

Tissue

30 mg

Cells

1–5 × 10⁶ cells

Feces / Gut Content

100 mg fresh / 20 mg lyophilized

Urine

50 μL

Exosomes

50 μL

Workflow

SERVICE WORKFLOW

Consultation

Study goals / design

Proposal & Quote

Targets / timeline / agreement

Sample Intake

Cold chain / pre-check / labeling

Experiment

Acquisition / QC

Delivery

Data + report + interpretation

Shipping notes: applicable to serum/plasma, various tissues, feces and gut content, urine, saliva, CSF, cells / cell culture medium, microbes and more — recommended amounts are for a single experiment, so please ship 2–3× the recommended amount; aspirate medium completely from cell samples and collect 10× more for small-volume cells such as immune cells; keep exosome protein concentration around 2 μg/μL; keep sampling sites consistent across groups for tissues; ship on dry ice or cold chain.

Analysis deliverables: QC correlation and PCA quality control · metabolite classification statistics · PCA / PLS-DA / OPLS-DA modeling · differential metabolite screening and hierarchical clustering · KEGG annotation, enrichment and pathway analysis · machine-learning (random forest) biomarker screening · full analysis report and raw data.

Metabolite Class Coverage (13 Classes / 685+ Compounds)

METABOLITE CLASS COVERAGE

Metabolite Class	Coverage	Representative Compounds
Amino Acids & Derivatives	123	20 proteinogenic amino acids, glutathione, sarcosine, kynurenine, etc.
Nucleotides & Derivatives	90	ATP/ADP/AMP, cAMP, purine and pyrimidine intermediates
Organic Acids & Derivatives	62	TCA-cycle intermediates, lactate, pyruvate, phenyllactate, etc.
Fatty Acids & Derivatives	63	Short- to long-chain fatty acids, hydroxy fatty acids, acyl-CoAs
Carbohydrates & Sugars	58	Glucose, fructose, sucrose, sugar alcohols and sugar phosphates
Benzenoids	40	Phenylalanine/tyrosine metabolites, phenols and aromatics
Hormones & Steroids	41	Sex hormones, corticosteroids, thyroid hormones
Bile Acids	33	Free and conjugated bile acids and their glycine/taurine conjugates
Carnitines & Acylcarnitines	36	Free carnitine, C2–C18 acylcarnitines
Vitamins & Derivatives	35	Water-soluble B vitamins, vitamins A/D/E/K and derivatives
Arachidonic Acid Metabolites	24	Prostaglandins, leukotrienes, HETEs and other eicosanoids
Organic Amines · Indoles & Heterocycles	50	Cholines, tryptamine, 5-HT metabolites, indole derivatives
Others (Flavonoids, Lipoids, AGEs, etc.)	31	Flavonoid monomers, lipoid molecules, AGEs, etc.

Applications

APPLICATIONS

Large-Cohort Metabolic ScreeningPlant Secondary-Metabolite ProfilingBiomarker Screening-to-ValidationTCM Quality EvaluationMulti-Species Broad-Coverage Testing

FAQ

How long does a Widely Targeted Metabolomics project take?

Mature targeted panels typically take 1-3 weeks; untargeted projects take 2-4 weeks (counted from sample receipt and QC approval). The exact timeline is confirmed in writing before project start, and progress is trackable on the OmicsHub cloud platform.

What if my sample amount is below the recommendation?

Please do not dilute your samples. Low-input samples can often be accommodated by adjusting the preparation workflow — tell us the actual amount and our technical team will assess feasibility and note adjustments in the study plan.

Can I add targets to an existing panel?

Yes. Mature panels support on-demand additions; novel analytes outside the list can be implemented through custom method development (requires standards and extra validation time) — see our Custom Targeted Metabolomics service.

What samples do you need for Widely Targeted Metabolomics?

Serum/plasma ≥100 μL (fasting, no haemolysis), tissue ≥50 mg (snap-frozen), cells ≥1×10⁶ (medium washed off), urine ≥200 μL. Keep fasting time consistent, aliquot into single-use volumes and avoid freeze–thaw. Full guidance: Technical Resources → Sample Guide.