WHOLE GENOME

Whole Genome Sequencing

Whole Genome Sequencing (WGS)

High-throughput whole-genome sequencing of samples, covering SNP, InDel and CNV detection and annotation to resolve genotype–phenotype associations at the genomic level. Suited to plant and animal breeding, population genetics, disease-associated variant discovery and microbial-genome research.

Technical Specifications

TECHNICAL SPECIFICATIONS

Sequencing Platform	Illumina high-throughput sequencing platform (PE150)
Analysis Content	Variant detection and annotation, population-genetics analysis, functional-gene mining
Sequencing depth	Tailored to the study objective (typically 10–30×)
Deliverables	raw data + standard analysis report

Sample Requirements

SAMPLE REQUIREMENTS

Genomic DNA

≥ 1 μg（OD260/280 1.8–2.0）

Tissue

≥ 100 mg

Whole Blood

≥ 1 mL (anticoagulated)

Quality requirements: intact, non-degraded DNA free of RNA and protein contamination; agarose-gel electrophoresis confirmation recommended.

Submission notes: ship DNA samples on dry ice or ice packs; specify species, reference-genome version and sample origin.

Workflow

WORKFLOW

Consultation

species / research objective

Proposal & Quote

Depth / timeline / agreement

Sample QC

concentration / purity / integrity

Library & Sequencing

library QC / sequencing

Analysis delivery

variant annotation + analysis report

FAQ

How long does a Whole Genome Sequencing project take?

From sample QC approval, standard timelines are 3-6 weeks (library prep, sequencing and bioinformatics included); large cohorts or deep custom analyses follow the confirmed study plan.

What if my samples fail QC?

Failed QC is reported immediately with reasons, and we discuss resampling or plan adjustments with you — sequencing never proceeds without qualification.

What bioinformatics is included? Can I add custom analyses?

Standard delivery covers QC, mapping/assembly, quantification, differential and functional enrichment analyses. Custom deep-dive and multi-omics integrative analyses are supported on request.

What samples do you need for Whole Genome Sequencing?

Total RNA ≥1 μg with RIN ≥7 (snap-frozen, dry ice); microbial and faecal samples ≥200 mg (sterile, −80 °C); whole blood for WGS ≥500 μL (EDTA only, never heparin). RNA integrity matters more than concentration. See Technical Resources → Sample Guide.