TMAO Metabolism Panel

Targeted List & Analysis Examples

Targeted List (partial)

NO.	Chinese Name	Abbr.	English Name	CAS No.
1	肉碱	Carn	Carnitine	541-15-1
2	胆碱	Cho	Choline	62-49-7
3	甜菜碱	BET	Betaine	107-43-7
4	肌酸肝	Cr	Creatinine	60-27-5
5	三甲胺	TMA	Trimethylamine	75-50-3
6	氧化三甲胺	TMAO	TrimethylamineN-oxide	1184-78-7

Full list available on request — contact our team.

Technical Specifications

TECHNICAL SPECIFICATIONS

Platform	UHPLC coupled with triple-quadrupole MS (UHPLC-MS/MS)
Ion Mode	Positive/negative ion switching acquisition
Quantification Unit	Standard-curve absolute quantification, ng/mL or ng/g
QC	Pooled QC samples inserted across the batch to monitor signal drift and stability

Sample Requirements

SAMPLE REQUIREMENTS

Serum / Plasma

50 μL

Tissue

30 mg

Cells

1–5 × 10⁶ cells

Feces / Gut Content

100 mg fresh / 20 mg lyophilized

Urine

50 μL

Exosomes

50 μL

Replicates: cell / microbial samples ≥6 replicates per group; animal samples ≥10 per group; clinical samples ≥50 per group with strictly controlled inclusion criteria. Sampling site, timing and handling must be consistent between experimental and control groups.

Shipping notes: applicable to serum/plasma, various tissues, feces and gut content, urine, saliva, cells / cell culture medium and more. Recommended amounts are for a single experiment — please ship 2–3× the recommended amount; keep sampling sites consistent across groups for tissue samples; ship on dry ice or cold chain.

Workflow

WORKFLOW

Consultation

Study goals / panel selection

Proposal & Quote

Targets / timeline / agreement

Sample Intake

Cold chain / pre-check / labeling

Experiment

Acquisition / QC

Delivery

Quantification + interpretation + raw data

Deliverables: precursor–product ratios and pathway-burden assessment · absolute-quantification table for each metabolite (concentration + units) · group-wise T-test and fold-change analysis · PCA / PLS-DA modeling and hierarchical clustering · KEGG annotation and pathway enrichment · full analysis report and raw data.

Analysis Examples

ANALYSIS EXAMPLES

Example figures are generated from real project data; actual figures and analyses depend on the detection panel and study plan.

T-test Bar Chart

All metabolites sorted by ascending p-value; grouped bars show group means ± SD with p-values annotated beside the bars.

Hierarchical Clustering Heatmap

Z-score normalized abundance patterns of all metabolites; rows ordered by hierarchical clustering — red for high, blue for low abundance.

Disease Association Bar Chart

Known metabolite–disease associations mined from HMDB and other databases, ranked by the number of associated metabolites.

Metabolite Abundance Analysis

Faceted boxplots of significantly different metabolites between groups; intergroup comparison by Welch's t-test.

Bar Chart Analysis

Per-sample bar charts of differential metabolites, visualizing group differences and individual dispersion.

References

REFERENCES

[1] WANG Z, KLIPFELL E, BENNETT B J, et al. Gut flora metabolism of phosphatidylcholine promotes cardiovascular disease[J]. Nature, 2011, 472(7341): 57-63.

[2] KOETH R A, WANG Z, LEVISON B S, et al. Intestinal microbiota metabolism of L-carnitine, a nutrient in red meat, promotes atherosclerosis[J]. Nature Medicine, 2013, 19(5): 576-585.

Applications

APPLICATIONS

Cardiovascular-disease risk researchGut-microbiota–host co-metabolismDietary choline/carnitine metabolism assessmentChronic-kidney-disease metabolism researchTMAO-intervention target evaluation

FAQ

How long does a TMAO Metabolism Panel project take?

Mature targeted panels typically take 1-3 weeks; untargeted projects take 2-4 weeks (counted from sample receipt and QC approval). The exact timeline is confirmed in writing before project start, and progress is trackable on the OmicsHub cloud platform.

What if my sample amount is below the recommendation?

Please do not dilute your samples. Low-input samples can often be accommodated by adjusting the preparation workflow — tell us the actual amount and our technical team will assess feasibility and note adjustments in the study plan.

Can I add targets to an existing panel?

Yes. Mature panels support on-demand additions; novel analytes outside the list can be implemented through custom method development (requires standards and extra validation time) — see our Custom Targeted Metabolomics service.

What samples do you need for TMAO Metabolism Panel?

Serum/plasma ≥100 μL (fasting, no haemolysis), tissue ≥50 mg (snap-frozen), cells ≥1×10⁶ (medium washed off), urine ≥200 μL. Keep fasting time consistent, aliquot into single-use volumes and avoid freeze–thaw. Full guidance: Technical Resources → Sample Guide.