Steroid Hormones Panel

Targeted List & Analysis Examples

Targeted List (partial)

NO.	Chinese Name	Abbr.	English Name	CAS No.
1	促肾上腺皮质激素	ACTH	Cosyntropin	16960-16-0
2	黄体刺激素	LH	Gonadorelin	9034-40-6
3	卵泡刺激素	FSH	Follicle-stimulatinghormone	9002-68-0
4	孕烯醇酮	P5	Pregnenolone	145-13-1
5	黄体酮	P4	Progesterone	57-83-0
6	皮质酮	Cortico	Corticosterone	50-22-6
7	醛固酮	Aldo	Aldosterone	52-39-1
8	17α-羟孕烯醇酮	17OH-P5	17α-Hydroxypregnenolone	387-79-1

Full list available on request — contact our team.

Technical Specifications

TECHNICAL SPECIFICATIONS

Platform	UHPLC coupled with triple-quadrupole MS (UHPLC-MS/MS)
Ion Mode	Positive/negative ion switching acquisition
Quantification Unit	Standard-curve absolute quantification, ng/mL or ng/g
QC	Pooled QC samples inserted across the batch to monitor signal drift and stability

Sample Requirements

SAMPLE REQUIREMENTS

Serum / Plasma

50 μL

Tissue

30 mg

Cells

1–5 × 10⁶ cells

Feces / Gut Content

100 mg fresh / 20 mg lyophilized

Urine

50 μL

Exosomes

50 μL

Replicates: cell / microbial samples ≥6 replicates per group; animal samples ≥10 per group; clinical samples ≥50 per group with strictly controlled inclusion criteria. Sampling site, timing and handling must be consistent between experimental and control groups.

Shipping notes: applicable to serum/plasma, various tissues, feces and gut content, urine, saliva, cells / cell culture medium and more. Recommended amounts are for a single experiment — please ship 2–3× the recommended amount; keep sampling sites consistent across groups for tissue samples; ship on dry ice or cold chain.

Workflow

WORKFLOW

Consultation

Study goals / panel selection

Proposal & Quote

Targets / timeline / agreement

Sample Intake

Cold chain / pre-check / labeling

Experiment

Acquisition / QC

Delivery

Quantification + interpretation + raw data

Deliverables: hormone-axis classification and precursor–product ratio analysis · absolute-quantification table for each metabolite (concentration + units) · group-wise T-test and fold-change analysis · PCA / PLS-DA modeling and hierarchical clustering · KEGG annotation and pathway enrichment · full analysis report and raw data.

Analysis Examples

ANALYSIS EXAMPLES

Example figures are generated from real project data; actual figures and analyses depend on the detection panel and study plan.

T-test Bar Chart

All metabolites sorted by ascending p-value; grouped bars show group means ± SD with p-values annotated beside the bars.

Hierarchical Clustering Heatmap

Z-score normalized abundance patterns of all metabolites; rows ordered by hierarchical clustering — red for high, blue for low abundance.

Disease Association Bar Chart

Known metabolite–disease associations mined from HMDB and other databases, ranked by the number of associated metabolites.

Metabolite Abundance Analysis

Faceted boxplots of significantly different metabolites between groups; intergroup comparison by Welch's t-test.

Bar Chart Analysis

Per-sample bar charts of differential metabolites, visualizing group differences and individual dispersion.

References

REFERENCES

[1] ŠIMKOVÁ M, KOLÁTOROVÁ L, DRAŠAR P, et al. An LC-MS/MS method for the simultaneous quantification of 32 steroids in human plasma[J]. Journal of Chromatography B, 2022, 1201: 123294.

[2] RASHID F, BAGHLA R, KALE P, et al. Absolute Quantification of Follicle Stimulating Hormone (FSH) Using Its Signature Peptides and Enzymatic Digestion in Human Serum by UPLC/LC-MS/MS[J]. Chromatographia, 2021, 84(8): 793-802.

Applications

APPLICATIONS

Endocrine-disease researchReproductive-hormone assessmentStress and cortisol researchHormone-replacement-therapy monitoring researchSteroid-biosynthesis pathway analysis

FAQ

How long does a Steroid Hormones Panel project take?

Mature targeted panels typically take 1-3 weeks; untargeted projects take 2-4 weeks (counted from sample receipt and QC approval). The exact timeline is confirmed in writing before project start, and progress is trackable on the OmicsHub cloud platform.

What if my sample amount is below the recommendation?

Please do not dilute your samples. Low-input samples can often be accommodated by adjusting the preparation workflow — tell us the actual amount and our technical team will assess feasibility and note adjustments in the study plan.

Can I add targets to an existing panel?

Yes. Mature panels support on-demand additions; novel analytes outside the list can be implemented through custom method development (requires standards and extra validation time) — see our Custom Targeted Metabolomics service.

What samples do you need for Steroid Hormones Panel?

Serum/plasma ≥100 μL (fasting, no haemolysis), tissue ≥50 mg (snap-frozen), cells ≥1×10⁶ (medium washed off), urine ≥200 μL. Keep fasting time consistent, aliquot into single-use volumes and avoid freeze–thaw. Full guidance: Technical Resources → Sample Guide.