qPCR Assay

Technical Specifications

TECHNICAL SPECIFICATIONS

Item	Details
Method	Real-time qPCR with SYBR Green or TaqMan probes, chosen by gene number and specificity requirements
Readout	Relative mRNA quantification (2^-ΔΔCt); absolute quantification with a standard curve is also available
Sample types	Tissue (RNAlater or snap-frozen), cell pellets, whole blood (PAXgene / EDTA), or total RNA (≥ 100 ng, RIN ≥ 7)
Controls	GAPDH / ACTB by default (species- and tissue-specific alternatives available); NTC and positive control on every plate
Deliverables	Amplification and melt curves, raw Ct table, relative expression figures and statistical results

Workflow

SERVICE WORKFLOW

Consultation

Target genes / species / sample types

Study design

Primer design and validation plan

Sample receipt

RNA QC (concentration / integrity)

RT & qPCR

cDNA synthesis and acquisition

Data analysis

Ct processing / relative quantification

Reporting

Data tables + figures + interpretation

Assay Menu

ASSAY MENU

Item	Details
Gene expression quantification	Relative expression between treatment and control groups, with biological replicate statistics
Primer design & validation	Species- and transcript-specific primers; single melt peak and 90–110% efficiency required before use
Absolute quantification	Standard curve over 10²–10⁸ copies, reported as copy number
miRNA / lncRNA quantification	Poly(A) tailing or stem-loop reverse transcription with dedicated primer sets
Multi-gene panels	Several targets measured in the same samples for pathway-level analysis

Sample Requirements

SAMPLE REQUIREMENTS

Tissue

≥ 30 mg, RNAlater or snap-frozen

Cells

≥ 1×10⁶, medium removed before freezing

Whole blood

≥ 500 μL (PAXgene / EDTA)

Total RNA

≥ 100 ng, RIN ≥ 7

Shipping: state species, tissue source and target gene list (with transcript IDs); ship frozen samples on dry ice.:

Applications

APPLICATIONS

qPCR validation of sequencing resultsPathway gene changes after drug treatmentGenotyping of transgenic / knockout modelsTranscript-level biomarker measurement in clinical samples

FAQ

How long does a qPCR Assay project take?

Standard projects take 1-3 weeks depending on complexity and sample size; projects involving cell modeling or multi-batch processing follow the confirmed study plan.

Can we provide our own reagents or antibodies?

Yes. You may supply key reagents/antibodies, or we can purchase them per the study plan. Please include datasheets and storage conditions for supplied materials.

Does the report include raw data?

Yes. Deliverables include raw records (membrane images, raw values, image files) together with processed statistical results — fully traceable.

What samples do you need for qPCR Assay?

ELISA serum/plasma ≥50 μL, WB lysate ≥20 μg protein, flow samples ≥100 μL (within 24 h), tissue ≥0.5 cm³ (4% PFA 24–48 h; OCT-embedded, dry ice). See Technical Resources → Sample Guide.