PRM 단백질체학

기술 사양

TECHNICAL SPECIFICATIONS

Platform	Orbitrap high-resolution MS in PRM mode
Quantification	Heavy-peptide internal standards; relative / absolute quantification
Throughput	Dozens to hundreds of peptides monitored per injection
Integration	Directly accepts candidate lists from discovery proteomics or literature

시료 요구량

SAMPLE REQUIREMENTS

세포

≥ 1 × 10⁶ 세포

조직

≥ 50 mg

혈청 / 혈장

≥ 20 μL

반복 수:그룹당 생물학적 반복 ≥3회, 검증 코호트에서는 더 많은 시료 수를 권장합니다.

배송 시 유의사항:-80°C에서 보관하고 드라이아이스로 배송하며, 펩타이드 설계를 위해 후보 단백질 목록(UniProt ID)을 제공해 주십시오.

분석 프로세스

WORKFLOW

목록 확정

후보 단백질 / 펩타이드 설계

제안서 및 견적

펩타이드 수 / 일정 / 계약

시료 접수

사전 점검 / 효소 분해

PRM 데이터 획득

표적 모니터링 / QC

결과 제공

정량 결과 + 분석 보고서

FAQ

How long does a PRM Proteomics project take?

Standard proteomics projects take 3-5 weeks depending on sample number and analysis depth; key milestones (sample QC, LC-MS/MS completion) are proactively synced with you.

How should proteomics samples be stored and shipped?

Store at -80°C and ship on dry ice; avoid repeated freeze-thaw cycles. Protease inhibitors are recommended for protein samples. A tailored Sample Collection Guide is provided before shipping.

How can DIA proteomics results be validated?

We recommend PRM targeted proteomics for candidate validation (antibody-free, highly reproducible), or classic assays such as Western Blot and ELISA, forming a "discovery → validation" closed loop.

What samples do you need for PRM Proteomics?

Cells ≥1×10⁶, tissue ≥50 mg, serum/plasma ≥50 μL, FFPE ≥4 sections (5 μm). Protein samples tolerate freeze–thaw poorly — aliquot so each tube is thawed once; for PTM work include matched controls and avoid phosphate buffers. See Technical Resources → Sample Guide.