번역후 변형 단백질체학

기술 사양

TECHNICAL SPECIFICATIONS

Platform	Orbitrap Astral / timsTOF high-resolution MS
Enrichment	TiO₂ / IMAC / antibody-based enrichment (selected by PTM type)
Analysis Content	Site identification, site quantification, motif analysis, kinase–substrate association
Deliverables	Raw data + standard analysis report + OmicsHub cloud archiving

시료 요구량

SAMPLE REQUIREMENTS

세포

≥ 1 × 10⁷ 세포

조직

≥ 100 mg

단백질 라이세이트

≥ 2 mg

반복 수:그룹당 생물학적 반복 ≥3–4회, 통계적 견고성을 위해 4회 이상을 권장합니다.

배송 시 유의사항:-80°C에서 보관하고 드라이아이스로 배송하며 동결-해동 반복을 피하십시오. 변형 단백질은 분해되기 쉬우므로 처리 시간을 최소화하고 적절한 효소 억제제를 첨가하십시오.

분석 프로세스

WORKFLOW

상담

PTM 유형 / 연구 목표

제안서 및 견적

농축 전략 / 일정 / 계약

시료 접수

사전 점검 / 단백질 정량

농축 및 데이터 획득

PTM 농축 / 고분해능 데이터 획득

결과 제공

부위 테이블 + 분석 보고서

FAQ

How long does a PTM Proteomics project take?

Standard proteomics projects take 3-5 weeks depending on sample number and analysis depth; key milestones (sample QC, LC-MS/MS completion) are proactively synced with you.

How should proteomics samples be stored and shipped?

Store at -80°C and ship on dry ice; avoid repeated freeze-thaw cycles. Protease inhibitors are recommended for protein samples. A tailored Sample Collection Guide is provided before shipping.

How can DIA proteomics results be validated?

We recommend PRM targeted proteomics for candidate validation (antibody-free, highly reproducible), or classic assays such as Western Blot and ELISA, forming a "discovery → validation" closed loop.

What samples do you need for PTM Proteomics?

Cells ≥1×10⁶, tissue ≥50 mg, serum/plasma ≥50 μL, FFPE ≥4 sections (5 μm). Protein samples tolerate freeze–thaw poorly — aliquot so each tube is thawed once; for PTM work include matched controls and avoid phosphate buffers. See Technical Resources → Sample Guide.