4D 단백질체학

기술 사양

TECHNICAL SPECIFICATIONS

Platform	timsTOF-series 4D MS (ion-mobility separation)
Acquisition mode	dia-PASEF data-independent acquisition
Analysis Content	Protein identification & quantification, differential analysis, functional enrichment, PPI networks
Best For	Large-cohort screening, low-input samples, deep-coverage needs

시료 요구량

SAMPLE REQUIREMENTS

세포

≥ 1 × 10⁶ 세포

조직

≥ 50 mg

혈청 / 혈장

≥ 50 μL

반복 수:그룹당 생물학적 반복 ≥3–4회, 대규모 코호트는 단일 배치로 처리하는 것을 권장합니다.

배송 시 유의사항:시료는 -80°C에서 보관하고 드라이아이스로 배송하며, 혈청 시료는 표준화된 채혈 및 처리 절차를 권장합니다.

분석 프로세스

WORKFLOW

상담

연구 목표 / 시료 유형

제안서 및 견적

분석 심도 / 일정 / 계약

시료 접수

사전 점검 / 단백질 정량

실험

DIA 데이터 획득 / QC

결과 제공

정량 매트릭스 + 분석 보고서

FAQ

How long does a 4D Proteomics project take?

Standard proteomics projects take 3-5 weeks depending on sample number and analysis depth; key milestones (sample QC, LC-MS/MS completion) are proactively synced with you.

How should proteomics samples be stored and shipped?

Store at -80°C and ship on dry ice; avoid repeated freeze-thaw cycles. Protease inhibitors are recommended for protein samples. A tailored Sample Collection Guide is provided before shipping.

How can DIA proteomics results be validated?

We recommend PRM targeted proteomics for candidate validation (antibody-free, highly reproducible), or classic assays such as Western Blot and ELISA, forming a "discovery → validation" closed loop.

What samples do you need for 4D Proteomics?

Cells ≥1×10⁶, tissue ≥50 mg, serum/plasma ≥50 μL, FFPE ≥4 sections (5 μm). Protein samples tolerate freeze–thaw poorly — aliquot so each tube is thawed once; for PTM work include matched controls and avoid phosphate buffers. See Technical Resources → Sample Guide.