광범위 표적 대사체학

기술 사양

TECHNICAL SPECIFICATIONS

Item	Details
Platform	UHPLC coupled with triple-quadrupole MS (UHPLC-MS/MS) · MRM mode with polarity switching
Quantification	Isotope internal standards + calibration-curve absolute quantification
Chromatography	20-minute gradient per injection
QC	QC samples inserted across the batch to monitor signal drift
Biological Replicates	Cell / microbial samples ≥6 replicates per group; animal samples ≥10 per group; clinical samples ≥50 per group with strictly controlled inclusion criteria. Sampling site, timing and handling must be consistent between experimental and control groups.

시료 요건

SAMPLE REQUIREMENTS

혈청 / 혈장

50 μL

조직

30 mg

세포

1–5 × 10⁶ 세포

분변 / 장 내용물

100 mg 신선 / 20 mg 동결건조

소변

50 μL

엑소좀

50 μL

분석 프로세스

SERVICE WORKFLOW

상담

연구 목표 / 설계

제안 & 견적

표적 / 일정 / 계약

시료 접수

콜드체인 / 사전 점검 / 라벨링

실험

데이터 획득 / QC

결과 제공

데이터 + 보고서 + 해석

발송 안내:혈청/혈장, 각종 조직, 분변 및 장 내용물, 소변, 타액, 뇌척수액, 세포 / 세포 배양 배지, 미생물 등에 적용됩니다. 권장량은 1회 실험 기준이므로 2–3배로 발송해 주십시오. 세포 시료는 배지를 완전히 제거하고, 면역세포처럼 부피가 적은 세포는 10배 더 수집해 주십시오. 엑소좀 단백질 농도는 약 2 μg/μL로 유지하고, 조직은 그룹 간 채취 부위를 동일하게 유지해 주십시오. 드라이아이스 또는 콜드체인으로 발송해 주십시오.

분석 제공 결과물:QC 상관성 및 PCA 품질관리 · 대사물질 분류 통계 · PCA / PLS-DA / OPLS-DA 모델링 · 차이 대사물질 선별 및 계층적 클러스터링 · KEGG 주석, 농축 및 경로 분석 · 머신러닝(랜덤 포레스트) 바이오마커 선별 · 전체 분석 보고서와 원시 데이터.

대사물질 분류 커버리지 (13개 분류 / 685종 이상 화합물)

METABOLITE CLASS COVERAGE

Metabolite Class	Coverage	Representative Compounds
Amino Acids & Derivatives	123	20 proteinogenic amino acids, glutathione, sarcosine, kynurenine, etc.
Nucleotides & Derivatives	90	ATP/ADP/AMP, cAMP, purine and pyrimidine intermediates
Organic Acids & Derivatives	62	TCA-cycle intermediates, lactate, pyruvate, phenyllactate, etc.
Fatty Acids & Derivatives	63	Short- to long-chain fatty acids, hydroxy fatty acids, acyl-CoAs
Carbohydrates & Sugars	58	Glucose, fructose, sucrose, sugar alcohols and sugar phosphates
Benzenoids	40	Phenylalanine/tyrosine metabolites, phenols and aromatics
Hormones & Steroids	41	Sex hormones, corticosteroids, thyroid hormones
Bile Acids	33	Free and conjugated bile acids and their glycine/taurine conjugates
Carnitines & Acylcarnitines	36	Free carnitine, C2–C18 acylcarnitines
Vitamins & Derivatives	35	Water-soluble B vitamins, vitamins A/D/E/K and derivatives
Arachidonic Acid Metabolites	24	Prostaglandins, leukotrienes, HETEs and other eicosanoids
Organic Amines · Indoles & Heterocycles	50	Cholines, tryptamine, 5-HT metabolites, indole derivatives
Others (Flavonoids, Lipoids, AGEs, etc.)	31	Flavonoid monomers, lipoid molecules, AGEs, etc.

적용 분야

APPLICATIONS

대규모 코호트 대사 스크리닝식물 이차대사물질 프로파일링바이오마커 스크리닝-검증 연계TCM 품질 평가다종 광범위 검출

FAQ

How long does a Widely Targeted Metabolomics project take?

Mature targeted panels typically take 1-3 weeks; untargeted projects take 2-4 weeks (counted from sample receipt and QC approval). The exact timeline is confirmed in writing before project start, and progress is trackable on the OmicsHub cloud platform.

What if my sample amount is below the recommendation?

Please do not dilute your samples. Low-input samples can often be accommodated by adjusting the preparation workflow — tell us the actual amount and our technical team will assess feasibility and note adjustments in the study plan.

Can I add targets to an existing panel?

Yes. Mature panels support on-demand additions; novel analytes outside the list can be implemented through custom method development (requires standards and extra validation time) — see our Custom Targeted Metabolomics service.

What samples do you need for Widely Targeted Metabolomics?

Serum/plasma ≥100 μL (fasting, no haemolysis), tissue ≥50 mg (snap-frozen), cells ≥1×10⁶ (medium washed off), urine ≥200 μL. Keep fasting time consistent, aliquot into single-use volumes and avoid freeze–thaw. Full guidance: Technical Resources → Sample Guide.