---
title: "Instrument Run"
lang: ko
url: https://www.gereallife.com/ko/services/resources-instrument.html
canonical: https://www.gereallife.com/ko/services/resources-instrument.html
entity: Shanghai Gereallife Biotech Co., Ltd
updated: 2026-11-02
---
KEY POINTS 
 
- PE150 mode, read length up to 2×300 bp
- up to 25 B reads per flow cell
- Q30 ≥ 85% (WGS)
- supports WGS / WES / RNA-seq / scRNA-seq 
 
 
 

## 01Sequencing Platforms

 

Covering NGS and third-generation sequencing (TGS), meeting every need from short to long reads and from bulk to single cell

### Illumina NGS Platform

short reads · high throughput · high accuracy

### Model

Illumina NovaSeq X Plus / NovaSeq 6000 / NextSeq 2000

### Technical Highlights

 
- PE150 mode, read length up to 2×300 bp
- up to 25 B reads per flow cell
- Q30 ≥ 85% (WGS)
- supports WGS / WES / RNA-seq / scRNA-seq 

### Applicable Omics

genomics (WGS/WES), transcriptomics (RNA-seq), single-cell sequencing

### PacBio TGS Platform

long reads · HiFi accuracy · direct methylation sequencing

### Model

PacBio Revio / Sequel IIe

### Technical Highlights

 
- HiFi reads: 15–25 kb average, >99.9% accuracy
- up to 90 Gb HiFi data per run
- direct DNA-methylation (5mC) detection
- ideal for complex-genome assembly and structural-variant detection 

### Applicable Omics

whole-genome sequencing (large genomes), full-length transcript sequencing, methylation sequencing

### Oxford Nanopore Platform

ultra-long reads · real-time sequencing · direct methylation detection

### Model

Oxford Nanopore PromethION 24 / MinION Mk1C

### Technical Highlights

 
- ultra-long reads: 30–50 kb average, >1 Mb maximum
- PCR-free direct sequencing with reduced bias
- real-time data output—analyze while sequencing
- suited to metagenomics, viral assembly and structural variation 

### Applicable Omics

metagenome sequencing, large-genome assembly, viral-diversity research

 
 
 

## 02Mass-Spectrometry Platforms

 

Top-tier Thermo Fisher and Bruker MS systems covering high-precision proteomics and metabolomics detection

### Thermo Orbitrap Astral

ultra-fast scanning · high sensitivity · 4D proteomics

### Technical Highlights

 
- scan rate: up to 200 Hz (Astral mass analyzer)
- resolution: up to 480,000 FWHM (Orbitrap)
- >12,000 proteins identified per DIA run
- dynamic range: > 5 orders of magnitude 

### Applications

4D proteomics (DIA/DDA/PRM), PTM analysis, large-cohort clinical studies

### Bruker timsTOF HT

ion-mobility separation · PASEF technology · ultra-high sensitivity

### Technical Highlights

 
- mobility resolution: up to 300–500 1/K0
- PASEF mode improves ion utilization 10–20×
- scan rate: up to 100 Hz MS/MS
- suited to ultra-low sample loads ( 

### Applications

low-input proteomics, single-cell proteomics, deep plasma proteomics

### Thermo Q Exactive HF-X

high resolution · accurate mass · for both metabolomics and proteomics

### Technical Highlights

 
- resolution: up to 240,000 FWHM (@ m/z 200)
- mass accuracy: 4 orders of magnitude
- supports DDA / DIA / PRM acquisition modes 

### Applications

metabolomics (LC-MS/MS untargeted + targeted), proteomics (DIA/DDA), PTM analysis

 
 
 

## 03Standard Run Procedure

 

Standardized procedure from library/sample loading to data output

### Run Preparation

library/sample QC recheck, flow-cell/column preparation

### Pooling & Loading

libraries pooled at equimolar ratios, diluted and loaded onto the flow cell

### Sequencing / Acquisition

run per preset protocol with real-time monitoring of Q30/resolution

### Real-Time QC

data quality checked every 12–24 h; parameters adjusted as needed

### Data Output

raw data (FASTQ/RAW/WIFF) transferred to storage servers

 
 
 

## 04Platform Parameter Comparison

 

| Platform | Read length / resolution | Throughput (per run) | Accuracy / mass accuracy | Run time |
| --- | --- | --- | --- | --- |
| Illumina NovaSeq X Plus | PE 2×150 bp | up to 25 B reads | Q30 ≥ 85% | approx. 24–48 h |
| PacBio Revio | HiFi 15–25 kb | up to 90 Gb | accuracy > 99.9% | approx. 24 h |
| Nanopore PromethION | ultra-long reads 30–50 kb | up to 150 Gb | accuracy ~98% (HAC model) | real-time (interruptible) |
| Orbitrap Astral | resolution 480,000 | ~200 Hz scan rate | mass accuracy 
 
 
 

## 05In-Run QC Monitoring

 

Key metrics monitored in real time during runs to ensure data meets delivery standards

### Sequencing QC Metrics

Q30 ≥ 85%

NGS Q30 (error rate ≤ 0.1%) ratio; below 80% triggers an alert and re-run assessment.

 
- Coverage uniformity: per-sample coverage CV 

### MS QC Metrics

MS1 resolution ≥ 60,000

MS1 resolution ≥ 60,000 for proteomics DIA; ≥ 35,000 for metabolomics.

 
- Retention-time drift: within batch 8,000 proteins 

### Alert & Response Mechanism

real-time monitoring · rapid response

QC reports are generated every 12 hours during runs; technicians act on the following rules:

 
- Yellow alert: Q30 drop within 3%—continue running with enhanced monitoring
- Orange alert: Q30 20%—pause and assess
- Red alert: severe deviation—terminate the run, troubleshoot and re-run 
 
 
 

## 06Pre-Run Checklist

 

### Pre-run checklist for sequencing samples

 
- library concentration ≥ 2 nM (Illumina standard); use EB buffer when diluting to working concentration
- library fragment distribution as expected (Agilent TapeStation), no significant adapter-dimer peaks
- libraries pooled at equimolar ratios to avoid over-representation of any sample
- clustering-efficiency forecast: expected reads per sample matching the study design
- flow-cell model matched to library type (NovaSeq X Plus uses X-series flow cells) 

### Pre-run checklist for MS samples

 
- protein/metabolite extracts fully depleted of host proteins/salts (lines flushed with LC-MS-grade water/acetonitrile)
- column equilibrated ≥ 30 min; retention-time stability RSD 
 
 
 

## Q&AFAQ

 How much data does one PE150 run produce? In PE150 mode read length reaches up to 2×300 bp and throughput is up to 25 B reads per flow cell, covering WGS / WES / RNA-seq / scRNA-seq. 
 Can you hold Q30 for whole-genome sequencing? The WGS standard is Q30 ≥ 85%, and runs are monitored in real time — dropping below 80% triggers an alert and a re-run assessment. 
 Which instruments do you run for long-read sequencing? Long-read work uses PacBio Revio / Sequel IIe and Oxford Nanopore PromethION 24 / MinION Mk1C. 
 My sample amount is very small — can I still do proteomics? Yes: the Bruker timsTOF HT is suited to ultra-low sample loads (< 100 ng) and is used for low-input, single-cell and deep plasma proteomics. 
 What does a library need before it can go on the instrument? Library concentration ≥ 2 nM (Illumina standard) diluted in EB buffer, expected fragment distribution with no significant adapter-dimer peaks, equimolar pooling, and a flow cell model matched to the library type. 
 
 
 
 
 
 Need study design? 
 

Free study design and sample assessment, response within 1 hour.

 Contact the team → 
 
 
 
 

 
 Call us： 400-660-8826 
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