---
title: "Widely Targeted Metabolomics"
lang: en
url: https://www.gereallife.com/en/services/widely-targeted-metabolomics.html
canonical: https://www.gereallife.com/en/services/widely-targeted-metabolomics.html
entity: Shanghai Gereallife Biotech Co., Ltd
updated: 2026-11-02
---
WIDELY-TARGETED METABOLOMICS 
 

# Widely Targeted Metabolomics

 

Widely-Targeted Metabolomics — Coverage of Discovery, Accuracy of Targeted Quantification

 

Between untargeted and targeted: triple-quadrupole MRM with isotope internal standards absolutely quantifies 685+ known metabolites in one run — retaining untargeted-level coverage while delivering targeted-level quantitative accuracy, ideal for studies that complete screening and verification in a single step.

 
 
 685+ Metabolites Quantified METABOLITES QUANTIFIED 13 Metabolite Classes METABOLITE CLASSES MRM Triple-Quad + Isotope IS MRM + ISOTOPE STANDARDS 

## Technical Specifications

 TECHNICAL SPECIFICATIONS 

| Item | Details |
| --- | --- |
| Platform | UHPLC coupled with triple-quadrupole MS (UHPLC-MS/MS) · MRM mode with polarity switching |
| Quantification | Isotope internal standards + calibration-curve absolute quantification |
| Chromatography | 20-minute gradient per injection |
| QC | QC samples inserted across the batch to monitor signal drift |
| Biological Replicates | Cell / microbial samples ≥6 replicates per group; animal samples ≥10 per group; clinical samples ≥50 per group with strictly controlled inclusion criteria. Sampling site, timing and handling must be consistent between experimental and control groups. |

 
 

## Sample Requirements

 SAMPLE REQUIREMENTS Serum / Plasma 50 μL Tissue 30 mg Cells 1–5 × 10⁶ cells Feces / Gut Content 100 mg fresh / 20 mg lyophilized Urine 50 μL Exosomes 50 μL 
 

## Workflow

 SERVICE WORKFLOW 1 Consultation Study goals / design 2 Proposal & Quote Targets / timeline / agreement 3 Sample Intake Cold chain / pre-check / labeling 4 Experiment Acquisition / QC 5 Delivery Data + report + interpretation Shipping notes: applicable to serum/plasma, various tissues, feces and gut content, urine, saliva, CSF, cells / cell culture medium, microbes and more — recommended amounts are for a single experiment, so please ship 2–3× the recommended amount; aspirate medium completely from cell samples and collect 10× more for small-volume cells such as immune cells; keep exosome protein concentration around 2 μg/μL; keep sampling sites consistent across groups for tissues; ship on dry ice or cold chain. Analysis deliverables: QC correlation and PCA quality control · metabolite classification statistics · PCA / PLS-DA / OPLS-DA modeling · differential metabolite screening and hierarchical clustering · KEGG annotation, enrichment and pathway analysis · machine-learning (random forest) biomarker screening · full analysis report and raw data. 
 

## Metabolite Class Coverage (13 Classes / 685+ Compounds)

 METABOLITE CLASS COVERAGE 

| Metabolite Class | Coverage | Representative Compounds |
| --- | --- | --- |
| Amino Acids & Derivatives | 123 | 20 proteinogenic amino acids, glutathione, sarcosine, kynurenine, etc. |
| Nucleotides & Derivatives | 90 | ATP/ADP/AMP, cAMP, purine and pyrimidine intermediates |
| Organic Acids & Derivatives | 62 | TCA-cycle intermediates, lactate, pyruvate, phenyllactate, etc. |
| Fatty Acids & Derivatives | 63 | Short- to long-chain fatty acids, hydroxy fatty acids, acyl-CoAs |
| Carbohydrates & Sugars | 58 | Glucose, fructose, sucrose, sugar alcohols and sugar phosphates |
| Benzenoids | 40 | Phenylalanine/tyrosine metabolites, phenols and aromatics |
| Hormones & Steroids | 41 | Sex hormones, corticosteroids, thyroid hormones |
| Bile Acids | 33 | Free and conjugated bile acids and their glycine/taurine conjugates |
| Carnitines & Acylcarnitines | 36 | Free carnitine, C2–C18 acylcarnitines |
| Vitamins & Derivatives | 35 | Water-soluble B vitamins, vitamins A/D/E/K and derivatives |
| Arachidonic Acid Metabolites | 24 | Prostaglandins, leukotrienes, HETEs and other eicosanoids |
| Organic Amines · Indoles & Heterocycles | 50 | Cholines, tryptamine, 5-HT metabolites, indole derivatives |
| Others (Flavonoids, Lipoids, AGEs, etc.) | 31 | Flavonoid monomers, lipoid molecules, AGEs, etc. |

 
 

## Applications

 APPLICATIONS 
 Large-Cohort Metabolic Screening Plant Secondary-Metabolite Profiling Biomarker Screening-to-Validation TCM Quality Evaluation Multi-Species Broad-Coverage Testing 
 

## FAQ

 FAQ 
 How long does a Widely Targeted Metabolomics project take? Mature targeted panels typically take 1-3 weeks; untargeted projects take 2-4 weeks (counted from sample receipt and QC approval). The exact timeline is confirmed in writing before project start, and progress is trackable on the OmicsHub cloud platform. What if my sample amount is below the recommendation? Please do not dilute your samples. Low-input samples can often be accommodated by adjusting the preparation workflow — tell us the actual amount and our technical team will assess feasibility and note adjustments in the study plan. Can I add targets to an existing panel? Yes. Mature panels support on-demand additions; novel analytes outside the list can be implemented through custom method development (requires standards and extra validation time) — see our Custom Targeted Metabolomics service. What samples do you need for Widely Targeted Metabolomics? Serum/plasma ≥100 μL (fasting, no haemolysis), tissue ≥50 mg (snap-frozen), cells ≥1×10⁶ (medium washed off), urine ≥200 μL. Keep fasting time consistent, aliquot into single-use volumes and avoid freeze–thaw. Full guidance: Technical Resources → Sample Guide . 
 
 

## Related Services

 RELATED SERVICES 
 

### Metabolic Flux & Isotope Tracing

Metabolic Flux & Isotope Tracing

 

### Targeted Metabolomics

Targeted Metabolomics

 

### Environmental Exposomics

Environmental Exposomics

 
 
 

Delivery timing: sample shipping, customs declaration and similar steps may extend turnaround; actual project timelines prevail.

 
 
 
 

## Request a Quote or Study Design

Tell us about your project — we reply within one business day (free assessment)

 
 
 
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## Start Your Multi-omics Project

 

Free study design · Interactive execution · In-depth data interpretation

 
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