---
title: "Western Blot"
lang: en
url: https://www.gereallife.com/en/services/western-blot.html
canonical: https://www.gereallife.com/en/services/western-blot.html
entity: Shanghai Gereallife Biotech Co., Ltd
updated: 2026-11-02
---
# Western Blot

 

Western Blot — Protein Expression Validation

 

A full Western-blot workflow—from protein extraction and quantification to electrophoresis, transfer, antibody incubation and imaging analysis—providing qualitative / semi-quantitative verification of target-protein expression level and molecular weight; a classic approach for gene-function studies and proteomics-result validation.

 
 
 End-to-end extraction to imaging analysis FULL WORKFLOW Densitometry housekeeping-normalized semi-quantification DENSITOMETRY Raw blot images complete raw-data delivery RAW IMAGES DELIVERED 

## Service Scope

 SERVICE SCOPE 

| Step | Notes |
| --- | --- |
| Protein extraction & quantification | Total-protein extraction from tissue/cells with BCA quantification |
| Electrophoresis & transfer | SDS-PAGE separation with wet / semi-dry transfer |
| Antibody incubation | Primary (purchasable on request) and secondary antibody incubation with ECL development |
| Imaging & analysis | Chemiluminescence imaging with housekeeping-normalized densitometry |

 
 

## Sample Requirements

 SAMPLE REQUIREMENTS Cells ≥ 1 × 10⁶ cells Tissue ≥ 50 mg Total protein ≥ 100 μg Replicates: ≥3 biological replicates per group to ensure valid semi-quantitative statistics. Submission notes: store samples at -80°C and ship on dry ice; provide the target-protein name, molecular weight and species; primary antibodies may be supplied by you or purchased by us. 
 

## Workflow

 WORKFLOW 1 Consultation Target protein / antibody confirmation 2 Proposal & Quote Targets / timeline / agreement 3 Sample Intake Pre-check / protein assay 4 Experiment electrophoresis / transfer / exposure 5 Delivery blot images + densitometric statistics 
 

## FAQ

 FAQ 
 How long does a Western Blot project take? Standard projects take 1-3 weeks depending on complexity and sample size; projects involving cell modeling or multi-batch processing follow the confirmed study plan. Can we provide our own reagents or antibodies? Yes. You may supply key reagents/antibodies, or we can purchase them per the study plan. Please include datasheets and storage conditions for supplied materials. Does the report include raw data? Yes. Deliverables include raw records (membrane images, raw values, image files) together with processed statistical results — fully traceable. What samples do you need for Western Blot? ELISA serum/plasma ≥50 μL, WB lysate ≥20 μg protein, flow samples ≥100 μL (within 24 h), tissue ≥0.5 cm³ (4% PFA 24–48 h; OCT-embedded, dry ice). See Technical Resources → Sample Guide . 
 
 

## Related Services

 RELATED SERVICES 
 

### ELISA

ELISA

 

### Biochemical Assays

Biochemical Assays

 

### Flow Cytometry

Flow Cytometry

 
 
 

Delivery timing: sample shipping, customs declaration and similar steps may extend turnaround; actual project timelines prevail.

 
 
 
 

## Request a Quote or Study Design

Tell us about your project — we reply within one business day (free assessment)

 
 
 
 Name 
 Organization 
 
 Phone or WeChat 
 Service of interest 
 Sample type, number of samples and aims (optional) 
 
 Submit 
 Used only for this enquiry; never shared. 
 
 
 
 
 

 
 

## Start Your Multi-omics Project

 

Free study design · Interactive execution · In-depth data interpretation

 
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 EMAIL market@gereallife.com 
 
 
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