--- title: "Single-cell RNA-seq" lang: en url: https://www.gereallife.com/en/services/scrna-seq.html canonical: https://www.gereallife.com/en/services/scrna-seq.html entity: Shanghai Gereallife Biotech Co., Ltd updated: 2026-11-02 --- SINGLE-CELL # Single-cell RNA-seq Single-cell RNA Sequencing (scRNA-seq) Built on dual platforms—10x Genomics Chromium X and MGI DNBelab—using microfluidic droplet technology for high-throughput single-cell capture and transcriptome library preparation, resolving cellular heterogeneity at single-cell resolution: cell clustering and annotation, differential expression, pseudotime and cell–cell interaction analysis. Suited to tumor-microenvironment, immunology, developmental and neuroscience research. Dual platforms Chromium X / DNBelab DUAL PLATFORMS Single-cell cell-heterogeneity resolution SINGLE-CELL RESOLUTION Full pipeline suspension prep to bioinformatics END-TO-END PIPELINE ## Technical Specifications TECHNICAL SPECIFICATIONS | Capture platform | 10x Genomics Chromium X / MGI DNBelab C-TaiM 4 | | --- | --- | | Sequencing Platform | Illumina high-throughput sequencing (PE150) | | Analysis Content | Cell clustering and annotation, differential expression, pseudotime, cell–cell communication | | Key QCs | Suspension viability, cell concentration and capture counts assessed throughout | ## Sample Requirements SAMPLE REQUIREMENTS Single-cell suspension viability ≥ 80% Cell concentration 700–1200 cells/μL Fresh tissue deliver ASAP on cold chain Key note: single-cell experiments are highly sensitive to sample freshness—book instrument time with our lab in advance and complete capture as soon as the suspension is prepared. Submission notes: ship fresh tissue on ice packs for delivery within 4 hours; keep cell suspensions ice-cold throughout; we recommend discussing the dissociation plan in advance. ## Workflow WORKFLOW 1 Consultation tissue type / research objective 2 Proposal & Quote cell count / timeline / agreement 3 Suspension prep dissociation / viability QC 4 Capture & sequencing library prep / sequencing / QC 5 Analysis delivery clustering & annotation + analysis report ## FAQ FAQ How long does a Single-cell RNA-seq project take? From sample QC approval, standard timelines are 3-6 weeks (library prep, sequencing and bioinformatics included); large cohorts or deep custom analyses follow the confirmed study plan. What if my samples fail QC? Failed QC is reported immediately with reasons, and we discuss resampling or plan adjustments with you — sequencing never proceeds without qualification. What bioinformatics is included? Can I add custom analyses? Standard delivery covers QC, mapping/assembly, quantification, differential and functional enrichment analyses. Custom deep-dive and multi-omics integrative analyses are supported on request. What samples do you need for Single-cell RNA-seq? Total RNA ≥1 μg with RIN ≥7 (snap-frozen, dry ice); microbial and faecal samples ≥200 mg (sterile, −80 °C); whole blood for WGS ≥500 μL (EDTA only, never heparin). RNA integrity matters more than concentration. See Technical Resources → Sample Guide . ## Related Services RELATED SERVICES ### Eukaryotic RNA-seq Eukaryotic RNA-seq ### Amplicon Sequencing Amplicon Sequencing ### Metagenomics Metagenomics Delivery timing: sample shipping, customs declaration and similar steps may extend turnaround; actual project timelines prevail. ## Request a Quote or Study Design Tell us about your project — we reply within one business day (free assessment) Name Organization Phone or WeChat Service of interest Sample type, number of samples and aims (optional) Submit Used only for this enquiry; never shared. ## Start Your Multi-omics Project Free study design · Interactive execution · In-depth data interpretation HOTLINE 400-660-8826 EMAIL market@gereallife.com Official Channels OFFICIAL CHANNELS Official Account WECHAT OFFICIAL ACCOUNT WeChat Service CUSTOMER SERVICE Xiaohongshu XIAOHONGSHU Call us： 400-660-8826 Scan to chat on WeChat