---
title: "qPCR Assay"
lang: en
url: https://www.gereallife.com/en/services/qpcr.html
canonical: https://www.gereallife.com/en/services/qpcr.html
entity: Shanghai Gereallife Biotech Co., Ltd
updated: 2026-11-02
---
qPCR 
 

# qPCR Assay

 

qPCR检测

 

Real-time quantitative PCR (SYBR Green or TaqMan probe) to measure target gene transcript levels, covering RNA extraction, reverse transcription, primer design and validation, and relative quantification.

 
 
 2 quantification chemistries SYBR GREEN & PROBE 3–5 working days TURNAROUND 3 technical replicates per sample TRIPLICATE 

## Technical Specifications

 TECHNICAL SPECIFICATIONS 

| Item | Details |
| --- | --- |
| Method | Real-time qPCR with SYBR Green or TaqMan probes, chosen by gene number and specificity requirements |
| Readout | Relative mRNA quantification (2^-ΔΔCt); absolute quantification with a standard curve is also available |
| Sample types | Tissue (RNAlater or snap-frozen), cell pellets, whole blood (PAXgene / EDTA), or total RNA (≥ 100 ng, RIN ≥ 7) |
| Controls | GAPDH / ACTB by default (species- and tissue-specific alternatives available); NTC and positive control on every plate |
| Deliverables | Amplification and melt curves, raw Ct table, relative expression figures and statistical results |

 
 

## Workflow

 SERVICE WORKFLOW 1 Consultation Target genes / species / sample types 2 Study design Primer design and validation plan 3 Sample receipt RNA QC (concentration / integrity) 4 RT & qPCR cDNA synthesis and acquisition 5 Data analysis Ct processing / relative quantification 6 Reporting Data tables + figures + interpretation 
 

## Assay Menu

 ASSAY MENU 

| Item | Details |
| --- | --- |
| Gene expression quantification | Relative expression between treatment and control groups, with biological replicate statistics |
| Primer design & validation | Species- and transcript-specific primers; single melt peak and 90–110% efficiency required before use |
| Absolute quantification | Standard curve over 10²–10⁸ copies, reported as copy number |
| miRNA / lncRNA quantification | Poly(A) tailing or stem-loop reverse transcription with dedicated primer sets |
| Multi-gene panels | Several targets measured in the same samples for pathway-level analysis |

 
 

## Sample Requirements

 SAMPLE REQUIREMENTS Tissue ≥ 30 mg, RNAlater or snap-frozen Cells ≥ 1×10⁶, medium removed before freezing Whole blood ≥ 500 μL (PAXgene / EDTA) Total RNA ≥ 100 ng, RIN ≥ 7 Shipping: state species, tissue source and target gene list (with transcript IDs); ship frozen samples on dry ice.: 
 

## Applications

 APPLICATIONS 
 qPCR validation of sequencing results Pathway gene changes after drug treatment Genotyping of transgenic / knockout models Transcript-level biomarker measurement in clinical samples 
 

## FAQ

 FAQ 
 How long does a qPCR Assay project take? Standard projects take 1-3 weeks depending on complexity and sample size; projects involving cell modeling or multi-batch processing follow the confirmed study plan. Can we provide our own reagents or antibodies? Yes. You may supply key reagents/antibodies, or we can purchase them per the study plan. Please include datasheets and storage conditions for supplied materials. Does the report include raw data? Yes. Deliverables include raw records (membrane images, raw values, image files) together with processed statistical results — fully traceable. What samples do you need for qPCR Assay? ELISA serum/plasma ≥50 μL, WB lysate ≥20 μg protein, flow samples ≥100 μL (within 24 h), tissue ≥0.5 cm³ (4% PFA 24–48 h; OCT-embedded, dry ice). See Technical Resources → Sample Guide . 
 
 

## Related Services

 RELATED SERVICES 
 

### ELISA

ELISA

 

### Biochemical Assays

Biochemical Assays

 

### Flow Cytometry

Flow Cytometry

 
 
 

Delivery timing: sample shipping, customs declaration and similar steps may extend turnaround; actual project timelines prevail.

 
 
 
 

## Request a Quote or Study Design

Tell us about your project — we reply within one business day (free assessment)

 
 
 
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## Start Your Multi-omics Project

 

Free study design · Interactive execution · In-depth data interpretation

 
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