---
title: "PRM Proteomics"
lang: en
url: https://www.gereallife.com/en/services/prm-proteomics.html
canonical: https://www.gereallife.com/en/services/prm-proteomics.html
entity: Shanghai Gereallife Biotech Co., Ltd
updated: 2026-11-02
---
PRM TARGETED 
 

# PRM Proteomics

 

Parallel Reaction Monitoring — Targeted Protein Quantification

 

Orbitrap PRM (parallel reaction monitoring) targets proteotypic peptides of candidate proteins, with heavy-labeled peptides enabling relative/absolute quantification. High selectivity, high reproducibility and antibody-free — an ideal alternative to high-throughput Western blot for candidate biomarker validation, closing the "discovery → validation" loop with discovery proteomics.

 
 
 PRM Proteotypic Peptide Targeting TARGETED PEPTIDES Heavy Peptides Relative / Absolute Quantification ISOTOPE-LABELED Antibody-Free High-Throughput WB Alternative ANTIBODY-FREE VALIDATION 

## Technical Specifications

 TECHNICAL SPECIFICATIONS 

| Platform | Orbitrap high-resolution MS in PRM mode |
| --- | --- |
| Quantification | Heavy-peptide internal standards; relative / absolute quantification |
| Throughput | Dozens to hundreds of peptides monitored per injection |
| Integration | Directly accepts candidate lists from discovery proteomics or literature |

 
 

## Sample Requirements

 SAMPLE REQUIREMENTS Cells ≥ 1 × 10⁶ cells Tissue ≥ 50 mg Serum / Plasma ≥ 20 μL Replicates: ≥3 biological replicates per group; larger sample sizes recommended for validation cohorts. Shipping notes: store at -80°C and ship on dry ice; provide the candidate protein list (UniProt IDs) for peptide design. 
 

## Workflow

 WORKFLOW 1 List Confirmation Candidates / peptide design 2 Proposal & Quote Peptide count / timeline / agreement 3 Sample Intake Pre-check / digestion 4 PRM Acquisition Targeted monitoring / QC 5 Delivery Quantification + analysis report 
 

## FAQ

 FAQ 
 How long does a PRM Proteomics project take? Standard proteomics projects take 3-5 weeks depending on sample number and analysis depth; key milestones (sample QC, LC-MS/MS completion) are proactively synced with you. How should proteomics samples be stored and shipped? Store at -80°C and ship on dry ice; avoid repeated freeze-thaw cycles. Protease inhibitors are recommended for protein samples. A tailored Sample Collection Guide is provided before shipping. How can DIA proteomics results be validated? We recommend PRM targeted proteomics for candidate validation (antibody-free, highly reproducible), or classic assays such as Western Blot and ELISA, forming a "discovery → validation" closed loop. What samples do you need for PRM Proteomics? Cells ≥1×10⁶, tissue ≥50 mg, serum/plasma ≥50 μL, FFPE ≥4 sections (5 μm). Protein samples tolerate freeze–thaw poorly — aliquot so each tube is thawed once; for PTM work include matched controls and avoid phosphate buffers. See Technical Resources → Sample Guide . 
 
 

## Related Services

 RELATED SERVICES 
 

### DIA Proteomics

DIA Proteomics

 

### PTM Proteomics

PTM Proteomics

 

### 4D Proteomics

4D Proteomics

 
 
 

Delivery timing: sample shipping, customs declaration and similar steps may extend turnaround; actual project timelines prevail.

 
 
 
 

## Request a Quote or Study Design

Tell us about your project — we reply within one business day (free assessment)

 
 
 
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## Start Your Multi-omics Project

 

Free study design · Interactive execution · In-depth data interpretation

 
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