--- title: "Folate Metabolism Panel" lang: en url: https://www.gereallife.com/en/services/folate.html canonical: https://www.gereallife.com/en/services/folate.html entity: Shanghai Gereallife Biotech Co., Ltd updated: 2026-11-02 --- FOLATE & ONE-CARBON METABOLISM # Folate Metabolism Panel Folate-Mediated One-Carbon Metabolism — Targeted Quantification Folate-mediated one-carbon metabolism supplies one-carbon units for nucleotide synthesis and methylation. Levels of intermediates such as tetrahydrofolate, 5-methyltetrahydrofolate and 5,10-methylenetetrahydrofolate directly relate to DNA synthesis and epigenetic modification, and are the target steps of antifolate drugs. This service absolutely quantifies folate-cycle intermediates for one-carbon-metabolism, epigenetics and nutritional-intervention research. 6 folate-cycle intermediates TARGET COMPOUNDS 2 links: nucleotide synthesis / methylation DOWNSTREAM PATHWAYS MRM Isotope-IS Absolute Quantification ABSOLUTE QUANTIFICATION ## Targeted List & Analysis Examples Targeted List & Analysis Examples Targeted List (partial) | NO. | Chinese Name | Abbr. | English Name | CAS No. | | --- | --- | --- | --- | --- | | 1 | 四氢叶酸 | THF | Tetrahydrofolicacid | 135-16-0 | | 2 | 二氢叶酸 | DHF | Dihydrofolicacid | 4033-27-6 | | 3 | 5,10-亚甲基四氢叶酸 | CH2-THF | 5,10-Methylenetetrahydrofolicacid | 31690-11-6 | | 4 | 5-甲基四氢叶酸 | CH3-THF | 5-Methyltetrahydrofolicacid | 134-35-0 | | 5 | S-腺苷甲硫氨酸 | SAM | S-Adenosylmethionine | 485-80-3 | | 6 | S-腺苷同型半胱氨酸 | SAH | S-Adenosylhomocysteine | 979-92-0 | Full list available on request — contact our team. ## Technical Specifications TECHNICAL SPECIFICATIONS | Platform | UHPLC coupled with triple-quadrupole MS (UHPLC-MS/MS) | | --- | --- | | Ion Mode | Positive/negative ion switching acquisition | | Quantification Unit | Standard-curve absolute quantification, ng/mL or ng/g | | QC | Pooled QC samples inserted across the batch to monitor signal drift and stability | ## Sample Requirements SAMPLE REQUIREMENTS Serum / Plasma 50 μL Tissue 30 mg Cells 1–5 × 10⁶ cells Feces / Gut Content 100 mg fresh / 20 mg lyophilized Urine 50 μL Exosomes 50 μL Replicates: cell / microbial samples ≥6 replicates per group; animal samples ≥10 per group; clinical samples ≥50 per group with strictly controlled inclusion criteria. Sampling site, timing and handling must be consistent between experimental and control groups. Shipping notes: applicable to serum/plasma, various tissues, feces and gut content, urine, saliva, cells / cell culture medium and more. Recommended amounts are for a single experiment — please ship 2–3× the recommended amount; keep sampling sites consistent across groups for tissue samples; ship on dry ice or cold chain. ## Workflow WORKFLOW 1 Consultation Study goals / panel selection 2 Proposal & Quote Targets / timeline / agreement 3 Sample Intake Cold chain / pre-check / labeling 4 Experiment Acquisition / QC 5 Delivery Quantification + interpretation + raw data Deliverables: one-carbon-intermediate distribution and methylation-potential assessment · absolute-quantification table for each metabolite (concentration + units) · group-wise T-test and fold-change analysis · PCA / PLS-DA modeling and hierarchical clustering · KEGG annotation and pathway enrichment · full analysis report and raw data. ## Analysis Examples ANALYSIS EXAMPLES Example figures are generated from real project data; actual figures and analyses depend on the detection panel and study plan. T-test Bar Chart All metabolites sorted by ascending p-value; grouped bars show group means ± SD with p-values annotated beside the bars. Hierarchical Clustering Heatmap Z-score normalized abundance patterns of all metabolites; rows ordered by hierarchical clustering — red for high, blue for low abundance. Disease Association Bar Chart Known metabolite–disease associations mined from HMDB and other databases, ranked by the number of associated metabolites. Metabolite Abundance Analysis Faceted boxplots of significantly different metabolites between groups; intergroup comparison by Welch's t-test. Bar Chart Analysis Per-sample bar charts of differential metabolites, visualizing group differences and individual dispersion. ## References REFERENCES [1] FRISO S, UDALI S, DE SANTIS D, et al. One-carbon metabolism and epigenetics[J]. Molecular Aspects of Medicine, 2017, 54: 28-36. [2] NANDANIA J, KOKKONEN M, EURO L, et al. Simultaneous measurement of folate cycle intermediates in different biological matrices using liquid chromatography-tandem mass spectrometry[J]. Journal of Chromatography B, 2018, 1092: 168-178. ## Applications APPLICATIONS One-carbon-metabolism pathway research Maternal–infant nutrition and birth-defect research Homocysteine-metabolism assessment Tumorigenesis and folate metabolism Methotrexate pharmacology research ## FAQ FAQ How long does a Folate Metabolism Panel project take? Mature targeted panels typically take 1-3 weeks; untargeted projects take 2-4 weeks (counted from sample receipt and QC approval). The exact timeline is confirmed in writing before project start, and progress is trackable on the OmicsHub cloud platform. What if my sample amount is below the recommendation? Please do not dilute your samples. Low-input samples can often be accommodated by adjusting the preparation workflow — tell us the actual amount and our technical team will assess feasibility and note adjustments in the study plan. Can I add targets to an existing panel? Yes. Mature panels support on-demand additions; novel analytes outside the list can be implemented through custom method development (requires standards and extra validation time) — see our Custom Targeted Metabolomics service. What samples do you need for Folate Metabolism Panel? Serum/plasma ≥100 μL (fasting, no haemolysis), tissue ≥50 mg (snap-frozen), cells ≥1×10⁶ (medium washed off), urine ≥200 μL. Keep fasting time consistent, aliquot into single-use volumes and avoid freeze–thaw. Full guidance: Technical Resources → Sample Guide . ## Related Services RELATED SERVICES ### Glucose Metabolism Panel Glucose Metabolism Panel ### Bile Acids Panel Bile Acids Panel ### SCFA & MCFA Panel SCFA & MCFA Panel Delivery timing: sample shipping, customs declaration and similar steps may extend turnaround; actual project timelines prevail. ## Request a Quote or Study Design Tell us about your project — we reply within one business day (free assessment) Name Organization Phone or WeChat Service of interest Sample type, number of samples and aims (optional) Submit Used only for this enquiry; never shared. ## Start Your Multi-omics Project Free study design · Interactive execution · In-depth data interpretation HOTLINE 400-660-8826 EMAIL market@gereallife.com Official Channels OFFICIAL CHANNELS Official Account WECHAT OFFICIAL ACCOUNT WeChat Service CUSTOMER SERVICE Xiaohongshu XIAOHONGSHU Call us： 400-660-8826 Scan to chat on WeChat