---
title: "SCFA & MCFA Panel"
lang: en
url: https://www.gereallife.com/en/services/fatty-acids.html
canonical: https://www.gereallife.com/en/services/fatty-acids.html
entity: Shanghai Gereallife Biotech Co., Ltd
updated: 2026-11-02
---
MEDIUM & LONG-CHAIN FATTY ACIDS 
 

# SCFA & MCFA Panel

 

Medium- and Long-Chain Fatty Acids Profiling

 

Free fatty acids are structural components of cell membranes and glycerolipids as well as precursors of energy supply and lipid signaling. This service covers short-, medium-, long- and very-long-chain fatty acids, plus selected branched, hydroxylated and rare unsaturated fatty acids, delivering a complete free-fatty-acid profile from a single injection. Chain-length and saturation composition is a direct readout of lipid synthesis, β-oxidation and membrane-fluidity regulation, suited to lipid-metabolism phenotyping, nutritional-intervention and metabolic-disease research.

 
 
 40+ target fatty acids TARGET COMPOUNDS 4 chains: short / medium / long / very long CHAIN LENGTH CLASSES MRM Isotope-IS Absolute Quantification ABSOLUTE QUANTIFICATION 

## Targeted List & Analysis Examples

 Targeted List & Analysis Examples Targeted List (partial) 
 

| NO. | Chinese Name | English Name | CAS No. |
| --- | --- | --- | --- |
| 1 | 癸酸 | Decanoicacid | 334-48-5 |
| 2 | 十一烷酸；篦麻油酸 | Undecanoicacid | 112-37-8 |
| 3 | 十二烷酸；月桂酸 | Dodecanoicacid | 143-07-7 |
| 4 | 十三烷酸 | Tridecanoicacid | 638-53-9 |
| 5 | 十四烷酸；肉豆蔻酸 | Tetradecanoicacid | 554-63-8 |
| 6 | 顺式-9-十四碳一烯酸/肉豆蔻油酸 | cis-9-Tetradecenoicacid | 544-64-9 |
| 7 | 反式-9-十四碳一烯酸/肉豆蔻反油酸 | Trans-9-Tetradecenoicacid | 50286-30-1 |
| 8 | 十五烷酸 | Pentadecanoicacid | 1002-84-2 |
| 9 | 顺式-10-十五碳一烯酸 | cis-10-Pentadecenoicacid | 84743-29-3 |
| 10 | 反式-10-十五碳一烯酸 | trans-10-Pentadecenoicacid | 321744-58-5 |
| 11 | 十六碳烷酸；棕榈酸 | Hexadecanoicacid | 57-10-3 |

 Full list available on request — contact our team. 
 

## Technical Specifications

 TECHNICAL SPECIFICATIONS 

| Platform | UHPLC coupled with triple-quadrupole MS (UHPLC-MS/MS) |
| --- | --- |
| Ion Mode | Positive/negative ion switching acquisition |
| Quantification Unit | Standard-curve absolute quantification, ng/mL or ng/g |
| QC | Pooled QC samples inserted across the batch to monitor signal drift and stability |

 
 

## Sample Requirements

 SAMPLE REQUIREMENTS Serum / Plasma 50 μL Tissue 30 mg Cells 1–5 × 10⁶ cells Feces / Gut Content 100 mg fresh / 20 mg lyophilized Urine 50 μL Exosomes 50 μL Replicates: cell / microbial samples ≥6 replicates per group; animal samples ≥10 per group; clinical samples ≥50 per group with strictly controlled inclusion criteria. Sampling site, timing and handling must be consistent between experimental and control groups. Shipping notes: applicable to serum/plasma, various tissues, feces and gut content, urine, saliva, cells / cell culture medium and more. Recommended amounts are for a single experiment — please ship 2–3× the recommended amount; keep sampling sites consistent across groups for tissue samples; ship on dry ice or cold chain. 
 

## Workflow

 WORKFLOW 1 Consultation Study goals / panel selection 2 Proposal & Quote Targets / timeline / agreement 3 Sample Intake Cold chain / pre-check / labeling 4 Experiment Acquisition / QC 5 Delivery Quantification + interpretation + raw data Deliverables: fatty-acid chain-length–saturation matrix with group statistics · absolute-quantification table for each metabolite (concentration + units) · group-wise T-test and fold-change analysis · PCA / PLS-DA modeling and hierarchical clustering · KEGG annotation and pathway enrichment · full analysis report and raw data. 
 

## Analysis Examples

 ANALYSIS EXAMPLES Example figures are generated from real project data; actual figures and analyses depend on the detection panel and study plan. T-test Bar Chart All metabolites sorted by ascending p-value; grouped bars show group means ± SD with p-values annotated beside the bars. Hierarchical Clustering Heatmap Z-score normalized abundance patterns of all metabolites; rows ordered by hierarchical clustering — red for high, blue for low abundance. Disease Association Bar Chart Known metabolite–disease associations mined from HMDB and other databases, ranked by the number of associated metabolites. Metabolite Abundance Analysis Faceted boxplots of significantly different metabolites between groups; intergroup comparison by Welch's t-test. Bar Chart Analysis Per-sample bar charts of differential metabolites, visualizing group differences and individual dispersion. 
 

## References

 REFERENCES [1] AL AIDAROS A, SHARMA C, LANGHANS C D, et al. Targeted metabolomic profiling of total fatty acids in human plasma by liquid chromatography-tandem mass spectrometry[J]. Metabolites, 2020, 10(10): 400. [2] CHEN Z, GAO Z, WU Y, et al. Development of a simultaneous quantitation for short-, medium-, long-, and very long-chain fatty acids in human plasma by 2-nitrophenylhydrazine-derivatization and liquid chromatography-tandem mass spectrometry[J]. Journal of Chromatography B, 2019, 1126-1127: 121771. 
 

## Technical Specifications

 TECHNICAL SPECIFICATIONS 

| Platform | UHPLC coupled with triple-quadrupole MS (UHPLC-MS/MS) |
| --- | --- |
| Ion Mode | Positive/negative ion switching acquisition |
| Quantification Unit | Standard-curve absolute quantification, ng/mL or ng/g |
| QC | Pooled QC samples inserted across the batch to monitor signal drift and stability |

 
 

## Sample Requirements

 SAMPLE REQUIREMENTS Serum / Plasma 50 μL Tissue 30 mg Cells 1–5 × 10⁶ cells Feces / Gut Content 100 mg fresh / 20 mg lyophilized Urine 50 μL Exosomes 50 μL Replicates: cell / microbial samples ≥6 replicates per group; animal samples ≥10 per group; clinical samples ≥50 per group with strictly controlled inclusion criteria. Sampling site, timing and handling must be consistent between experimental and control groups. Shipping notes: applicable to serum/plasma, various tissues, feces and gut content, urine, saliva, cells / cell culture medium and more. Recommended amounts are for a single experiment — please ship 2–3× the recommended amount; keep sampling sites consistent across groups for tissue samples; ship on dry ice or cold chain. 
 

## Workflow

 WORKFLOW 1 Consultation Study goals / panel selection 2 Proposal & Quote Targets / timeline / agreement 3 Sample Intake Cold chain / pre-check / labeling 4 Experiment Acquisition / QC 5 Delivery Quantification + interpretation + raw data Deliverables: total SCFA content and molar-proportion statistics · absolute-quantification table for each metabolite (concentration + units) · group-wise T-test and fold-change analysis · PCA / PLS-DA modeling and hierarchical clustering · KEGG annotation and pathway enrichment · full analysis report and raw data. 
 

## Targeted List & Analysis Examples

 Targeted List & Analysis Examples Targeted List (partial) 
 

| NO. | Chinese Name | English Name | CAS No. |
| --- | --- | --- | --- |
| 1 | 乙酸 | Aceticacid | 64-19-7 |
| 2 | 丙酸 | Propionicacid | 79-09-4 |
| 3 | 丁酸 | Butyricacid | 107-92-6 |
| 4 | 2-甲基丙酸（异丁酸） | 2-Methylpropionicacid | 79-31-2 |
| 5 | 戊酸（缬草酸） | Pentanoicacid | 109-52-4 |
| 6 | 3-甲基丁酸（异戊酸） | 3-Methylbutyricacid | 503-74-2 |
| 7 | 2-甲基丁酸 | 2-Methylbutyricacid | 142-62-1 |
| 8 | 2-甲基戊酸 | 2-Methylpentanoicacid | 116-53-0 |
| 9 | 4-甲基戊酸（异己酸） | 4-Methylvalericacid | 646-07-1 |
| 10 | 己酸 | Hexanoicacid | 142-62-1 |
| 11 | 庚酸 | Heptanoicacid | 111-14-8 |

 Full list available on request — contact our team. 
 

## Analysis Examples

 ANALYSIS EXAMPLES Example figures are generated from real project data; actual figures and analyses depend on the detection panel and study plan. T-test Bar Chart All metabolites sorted by ascending p-value; grouped bars show group means ± SD with p-values annotated beside the bars. Hierarchical Clustering Heatmap Z-score normalized abundance patterns of all metabolites; rows ordered by hierarchical clustering — red for high, blue for low abundance. Disease Association Bar Chart Known metabolite–disease associations mined from HMDB and other databases, ranked by the number of associated metabolites. Metabolite Abundance Analysis Faceted boxplots of significantly different metabolites between groups; intergroup comparison by Welch's t-test. Bar Chart Analysis Per-sample bar charts of differential metabolites, visualizing group differences and individual dispersion. 
 

## References

 REFERENCES [1] KOH A, DE VADDER F, KOVATCHEVA-DATCHARY P, et al. From Dietary Fiber to Host Physiology: Short-Chain Fatty Acids as Key Bacterial Metabolites[J]. Cell, 2016, 165(6): 1332-1345. [2] CANANI R B, DI COSTANZO M, LEONE L, et al. Potential beneficial effects of butyrate in intestinal and extraintestinal diseases[J]. World Journal of Gastroenterology, 2011, 17(12): 1519-1528. 
 

## Applications

 APPLICATIONS 
 Inflammation and immunometabolism research Cardiovascular risk assessment Nutritional fatty-acid profiling Gut-microbiota metabolism research Membrane-lipid composition analysis 
 

## FAQ

 FAQ 
 How long does a SCFA & MCFA Panel project take? Mature targeted panels typically take 1-3 weeks; untargeted projects take 2-4 weeks (counted from sample receipt and QC approval). The exact timeline is confirmed in writing before project start, and progress is trackable on the OmicsHub cloud platform. What if my sample amount is below the recommendation? Please do not dilute your samples. Low-input samples can often be accommodated by adjusting the preparation workflow — tell us the actual amount and our technical team will assess feasibility and note adjustments in the study plan. Can I add targets to an existing panel? Yes. Mature panels support on-demand additions; novel analytes outside the list can be implemented through custom method development (requires standards and extra validation time) — see our Custom Targeted Metabolomics service. What samples do you need for SCFA & MCFA Panel? Serum/plasma ≥100 μL (fasting, no haemolysis), tissue ≥50 mg (snap-frozen), cells ≥1×10⁶ (medium washed off), urine ≥200 μL. Keep fasting time consistent, aliquot into single-use volumes and avoid freeze–thaw. Full guidance: Technical Resources → Sample Guide . 
 
 

## Related Services

 RELATED SERVICES 
 

### Glucose Metabolism Panel

Glucose Metabolism Panel

 

### Bile Acids Panel

Bile Acids Panel

 

### Oxylipins Panel

Oxylipins Panel

 
 
 

Delivery timing: sample shipping, customs declaration and similar steps may extend turnaround; actual project timelines prevail.

 
 
 
 

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