---
title: "4D Proteomics"
lang: en
url: https://www.gereallife.com/en/services/4d-proteomics.html
canonical: https://www.gereallife.com/en/services/4d-proteomics.html
entity: Shanghai Gereallife Biotech Co., Ltd
updated: 2026-11-02
---
# 4D Proteomics

 

4D Proteomics — Ion Mobility Enhanced DIA

 

Building on the three classical dimensions — retention time, m/z and ion intensity — 4D proteomics adds ion mobility (CCS) as a fourth separation dimension, markedly improving peptide selectivity and identification depth in complex samples. Centered on DIA acquisition with timsTOF platforms, it combines high throughput, high reproducibility and deep coverage, ideal for large-cohort proteomics and low-abundance samples.

 
 
 4D Ion-Mobility 4th Dimension ION MOBILITY DIMENSION DIA Data-Independent Acquisition DEEP & REPRODUCIBLE High Throughput Stable Large-Cohort Delivery COHORT-READY 

## Technical Specifications

 TECHNICAL SPECIFICATIONS 

| Platform | timsTOF-series 4D MS (ion-mobility separation) |
| --- | --- |
| Acquisition mode | dia-PASEF data-independent acquisition |
| Analysis Content | Protein identification & quantification, differential analysis, functional enrichment, PPI networks |
| Best For | Large-cohort screening, low-input samples, deep-coverage needs |

 
 

## Sample Requirements

 SAMPLE REQUIREMENTS Cells ≥ 1 × 10⁶ cells Tissue ≥ 50 mg Serum / Plasma ≥ 50 μL Replicates: ≥3–4 biological replicates per group; large cohorts are recommended to be processed in unified batches. Shipping notes: store samples at -80°C and ship on dry ice; standardized blood collection and processing are recommended for serum samples. 
 

## Workflow

 WORKFLOW 1 Consultation Study goals / sample types 2 Proposal & Quote Depth / timeline / agreement 3 Sample Intake Pre-check / protein assay 4 Experiment DIA acquisition / QC 5 Delivery Quantification matrix + analysis report 
 

## FAQ

 FAQ 
 How long does a 4D Proteomics project take? Standard proteomics projects take 3-5 weeks depending on sample number and analysis depth; key milestones (sample QC, LC-MS/MS completion) are proactively synced with you. How should proteomics samples be stored and shipped? Store at -80°C and ship on dry ice; avoid repeated freeze-thaw cycles. Protease inhibitors are recommended for protein samples. A tailored Sample Collection Guide is provided before shipping. How can DIA proteomics results be validated? We recommend PRM targeted proteomics for candidate validation (antibody-free, highly reproducible), or classic assays such as Western Blot and ELISA, forming a "discovery → validation" closed loop. What samples do you need for 4D Proteomics? Cells ≥1×10⁶, tissue ≥50 mg, serum/plasma ≥50 μL, FFPE ≥4 sections (5 μm). Protein samples tolerate freeze–thaw poorly — aliquot so each tube is thawed once; for PTM work include matched controls and avoid phosphate buffers. See Technical Resources → Sample Guide . 
 
 

## Related Services

 RELATED SERVICES 
 

### DIA Proteomics

DIA Proteomics

 

### PTM Proteomics

PTM Proteomics

 

### PRM Proteomics

PRM Proteomics

 
 
 

Delivery timing: sample shipping, customs declaration and similar steps may extend turnaround; actual project timelines prevail.

 
 
 
 

## Request a Quote or Study Design

Tell us about your project — we reply within one business day (free assessment)

 
 
 
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## Start Your Multi-omics Project

 

Free study design · Interactive execution · In-depth data interpretation

 
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